Detail of "5381-99-7"

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Capped synthetic RNA preparation by simplified and efficient method, influenza virus endonuclease aptamers, and virus prevention and treatment

Capped synthetic RNA preparation by simplified and efficient method, influenza virus endonuclease aptamers, and virus prevention and treatment. Benseler, Fritz; Cole, James L.; Kuo, Lawrence C.; Olsen, David B.Several substances are used for example 5381-99-7 and 175452-35-4 which are their cas registry numbers. ( Merck and Co., Inc.; Benseler, Fritz; Cole, James L.; Kuo, Lawrence C.; Olsen, David B., USA). PCT Int. Appl. WO 9640159 A1 19 Dec 1996, 38 pp. DESIGNATED STATES: W: CA, JP, US; RW: AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE. (English). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: A61K031-70. ICS: C07H021-04. APPLICATION: WO 1996-US8394 3 Jun 1996. PRIORITY: US 1995-480068 7 Jun 1995. DOCUMENT TYPE: Patent CA Section: 1 (Pharmacology) Section cross-reference(s): 7, 10, 33 A simplified and efficient method is provided for making synthetic capped RNAs. Esp., the phosphitylation agent, 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one is used. RNA or analogs are phosphitylated or phosphorylated, then oxidn., deprotection, and hydrolysis steps follow. RNA phosphates are finally capped. These compds. serve as substrates for the virally encoded endonuclease assocd. with influenza virus. We are able to assay for this unique and specific viral activity of cleavage of a capped RNA in vitro. Therefore, screening of inhibitors of this activity is possible. In addn., short non-extendible (due to their length or because of the modification of the 3'-end of the oligo, i.e. 3'-dA) RNAs are potent inhibitors of the cleavage of capped RNAs by influenza endonuclease. Finally, these compds. may be used to investigate viral and cellular mechanisms of transcription/translation or mRNA maturation. .

A Synthesis of Dolichyl Phosphorofluoridate

A Synthesis of Dolichyl Phosphorofluoridate. Sizova, O. V.; Mal'tsev, S. D.; Shibaev, V. N. (Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia). Russian Journal of Bioorganic Chemistry (Translation of Bioorganicheskaya Khimiya), 29(4), 387-389 (English) 2003 MAIK Nauka/Interperiodica Publishing. CODEN: RJBCET.Several substances with their cas registry numbers 5381-99-7 and 650609-75-9 may be metioned in this study. ISSN: 1068-1620. DOCUMENT TYPE: Journal CA Section: 30 (Terpenes and Terpenoids) Section cross-reference(s): 29 An improved method for the synthesis of dolichyl hydrogen phosphonate was developed using 2-chloro-4H-1,3,2-benzodioxaphosphorin-4-one, a.k.a salicyl phosphorochloridite, as a reagent. Dolichyl phosphorofluoridate was for the first time synthesized from dolichyl hydrogen phosphonate phosphonate by its treatment with chlorotrimethylsilane,followed by oxidn. with iodine in the presence of fluoride in pyridine. .