Reference

Induction of hypericins and hyperforin in Hypericum perforatum L

Induction of hypericins and hyperforin in Hypericum perforatum L. in response to biotic and chemical elicitors. Sirvent, Tara; Gibson, Donna (Department of Plant Pathology, Cornell University, Ithaca, NY, USA). Physiological and Molecular Plant Pathology, 60(6), 311-320 (English) 2002 Elsevier Science Ltd. CODEN: PMPPEZ. ISSN: 0885-5765. DOCUMENT TYPE: Journal CA Section: 11 (Plant Biochemistry) Hypericum perforatum L. produces hyperforins, a family of antimicrobial acylphloroglucinols; and hypericins, a family of phototoxic anthraquinones exhibiting anti-microbial, anti-viral, and anti-herbivore properties in vitro. To det. whether these secondary metabolites are part of the specific plant defense systems that are mediated by Me jasmonate or salicylic acid, we used meristem cultures to assess the effects of exposure to exogenous application of these chem. elicitors. Levels of hypericins in plant tissue increased in response to both elicitor treatments; total hypericin levels increased as much as 3.3 times control levels when treated with 200 mM Me jasmonate for 14 days. Increased hyperforin concns. were detected when plantlets were treated with 1 m M salicylic acid or 50 mM Me jasmonate. For assessing responses to a biotic elicitor, greenhouse-grown plant materials were inoculated with the plant pathogen, Colletotrichum gloeosporioides. Levels of hypericins increased twice as much as the control when inoculated with 1 ′ 104 spores per mL; higher doses of spores overwhelmed the plant defenses. The elevation of hypericins and hyperforin in response to chem.In this experiment, several chemicals are used like 54328-09-5 and 1211-29-6 and biotic elicitors suggests that these secondary metabolites are components in the inducible plant defense responses of H. perforatum. .