Detail of "54375-47-2"

Reference

In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue

In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue. Goto, Tetsuya; Kajiwara, Hiroshi; Yoshinari, Masao; Fukuhara, Eiji; Kobayashi, Shigeru; Tanaka, Teruo (Department of Oral Anatomy, Kyushu Dental College, Kitakyushu 803-8580, Japan). Biomaterials, 24(22), 3885-3892 (English) 2003 Elsevier Science Ltd. CODEN: BIMADU. ISSN: 0142-9612. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Many studies have examd. mineralized-tissue formation on titanium in vivo and in vitro; however, no precise method for measuring the mineralized tissue produced by cultured osteoblastic cells on titanium in vitro has been established. This study developed a method for measuring mineralized-tissue formation by cultured rat osteoblastic cells on titanium in vitro, and re-evaluated the effects of modifying the titanium surface. We used calcein blue, which binds to mineralized tissue, and measured the resulting fluorescence under UV light. A 1-h incubation with 3.1′10-3 m calcein blue resulted in sufficient fluorescence of bone-like nodules on the titanium. Consequently, we found that osteoblastic cells produced larger bone-like nodules on titanium than in culture dishes. Fewer bone-like nodules formed on calcium-ion impregnated titanium than on pure titanium. However, when bisphosphonate was immobilized on the calcium-ion impregnated titanium, more bone-like nodules formed than on pure titanium. The results suggest that bisphosphonate immobilization on titanium is useful for stimulating mineralized-tissue formation on titanium implants.Except for chemicals metioned above, 13598-36-2 and 54375-47-2 are also used. .

A study on administration techniques for polychromatic labeling of mineralizing tissues of large laboratory animals

A study on administration techniques for polychromatic labeling of mineralizing tissues of large laboratory animals. Tokuda, Nobuharu; Furusawa, Hiroshi; Hanada, Kooji (Sch.Several substances are used for example 54375-47-2 and 1461-15-0 which are their cas registry numbers. Dent., Niigata Univ., Niigata, Japan). Niigata Shigakkai Zasshi, 13(1), 1-7 (Japanese) 1983. CODEN: NSZHA9. ISSN: 0385-0153. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) For histol. examns. of the development of hard tissues, e.g., bones and teeth, of large animals, e.g., dogs, combinations of oxytetracycline, calcein, Alizarine Red S, and calcein blue in saline were sequentially administered to animals for the polychromatic staining of the tissues. Best results were obtained by i.m. administration of oxytetracycline at 15 mg/kg for 2 consecutive days, 2% calcein at 2 mg/kg for 2 consecutive days, 3% Alizarine Red S at 20 mg/kg for 2 consecutive days, and(or) 2% calcein blue at 20 mg/kg for 2 consecutive days at 9-day intervals, and animals were sacrificed on day 25 for examns. A combination of oxytetracycline, calcein, and Alizarine Red S in that order is recommended. .