Reference

Stroma-free, non-heme protein-free hemoglobin

Stroma-free, non-heme protein-free hemoglobin. Tye, Ross W. (United States Dept. of the Army, USA). U.S. US 4473494 A 25 Sep 1984, 4 pp. (English). (United States of America). CODEN: USXXAM. CLASS: IC: A61K035-14; A61K037-04; C07C103-52. NCL: 260112000B. APPLICATION: US 83-497455 4 May 1983. DOCUMENT TYPE: Patent CA Section: 63 (Pharmaceuticals) Stroma-free, nonheme protein-free Hb is prepd. by using a 10-fold excess of Zn ion to promote pptn. of Zn-bound Hb complex followed by membrane ultrafiltration of the compds. from the filtrate fluid medium. Thus, erythrocytes are washed with saline and centrifuged to remove plasma proteins and dild. with 20 vols. of hypotonic buffer. The stroma-free Hb is collected as filtrate and is further filtered through a mol. wt. 1,000,000 millipore filter to remove viruses and protein aggregates. Further adjustment of the erythroycte content to 100 mM NaCl, addn. of 10-20 mol Zn(OAc)2 [557-34-6]/mol of Hb, removal of the supernatant, washing of the concd. Zn-Hb complex with saline, addn. of 1M EDTA [60-00-4]/1M Zn(OAc)2 and removal of Hb on a millipore filter, concn. of Hb by dialysis with EDTA and with saline leads to stroma-free nonheme protein-free Hb.

Thermal instabilities in vinyl acetate synthesis in packed bed reactors

Thermal instabilities in vinyl acetate synthesis in packed bed reactors. Ulrich, Detlef; Adler, Roland; Jankowski, Harry (VEB Chem. Werke Buna, Schkopau DDR-4212, Ger. Dem. Rep.). Chem. Tech. (Leipzig), 36(6), 239-43 (German) 1984. CODEN: CHTEAA. ISSN: 0045-6519. DOCUMENT TYPE: Journal CA Section: 35 (Chemistry of Synthetic High Polymers) Section cross-reference(s): 45 The runaway operating states occurring in the synthesis of vinyl acetate [108-05-4] from C2H2 [74-86-2] and HOAc [64-19-7] by using Zn(OAc)2 [557-34-6]-activated carbon as catalysts in packed bed reactors were investigated in lab. flow tubular reactors. Addnl. measurement of adsorption showed that decompn. of Zn(OAc)2 into ZnO effected the runaways. The model elaborated for the reaction mechanism of runaways permitted an illustration of the performance of packed bed reactors used for vinyl acetate synthesis within the sensitive parameter limits.