Reference

High sensitivity amino acid analysis using DABS-Cl precolumn derivatization method

High sensitivity amino acid analysis using DABS-Cl precolumn derivatization method. Knecht, Rene; Chang, Jui Yoa (Pharm. Res. Lab., CIBA-GEIGY Ltd. Basel, Basel CH-4002, Switz.). Adv. Methods Protein Microsequence Anal., 56-61. Edited by: Wittmann-Liebold, Brigitte; Salnikow, Johann; Erdmann, Volker A. Springer: Berlin, Fed. Rep. Ger. (English) 1986. CODEN: 55PPAG. DOCUMENT TYPE: Conference CA Section: 9 (Biochemical Methods) Reagents and equipment, dimethylaminoazobenzene sulfonyl chloride (DABS-Cl)-derivatized amino acid stds., prepn. of peptide and protein samples, acid hydrolysis, dabsylation, and HPLC quant. anal. of DABS-amino acids are discussed.Except for chemicals metioned above, 56512-49-3 is also used. .

Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes

Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes. Benson, Scott C.; Menchen, Steven M.; Upadhya, Krishna G. (Applera Corporation, USA). PCT Int. Appl. WO 2006031851 A1 23 Mar 2006, 61 pp. DESIGNATED STATES: W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SM, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW; RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, CY, DE, DK, ES, FI, FR, GA, GB, GR, IE, IS, IT, LU, MC, ML, MR, NE, NL, PT, SE, SN, TD, TG, TR. (English). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: G01N033-542. ICS: C09B011-00. APPLICATION: WO 2005-US32659 14 Sep 2005. PRIORITY: US 2004-609273P 14 Sep 2004. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 3, 34, 41 Dark quencher constructs, termed "multi-chromophoric quenchers" are described herein that comprise at least two dark quenching moieties, which can be the same or different, linked together by at least one multivalent linking moiety. The structure of the multi-chromophoric quenchers can be varied to selectively enhance quenching within a specific range of reporter emission wavelengths. This can be accomplished by linking together, into a single mol., two or more identical quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to quench a broader range of reporter emission wavelengths than previously possible. This can be accomplished by linking together, into a single mol., two or more different quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to simultaneously broaden the absorption range and increase the total absorption within the absorption range. This can be done by combining the two concepts described above. In other words, multiple types of quenching moieties can be employed to increase the absorption range and a multiple no. of each type of quenching moiety can be used to increase the total absorptivity within the absorption range. There are some commonly used reagents like 56512-49-3 in this article. The multi-chromophoric quenchers can be tethered to probes for biomols., insol. supports and/or fluorescent dyes for use in a wide variety of biomol. assays. N,N'-bis dabsyl-ornithine NHS ester, prepd. from L-ornithine Me ester and dabsyl NHS, was conjugated to a 5'-derivatized 3'-FAM oligonucleotide to prep. a multi-chromophoric quencher construct. .