Detail of "56548-64-2"

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Mutagenicity evaluation of the commercial product CI Disperse Blue 291 using different protocols of the Salmonella assay

Mutagenicity evaluation of the commercial product CI Disperse Blue 291 using different protocols of the Salmonella assay. Umbuzeiro, Gisela de Aragao; Freeman, Harold; Warren, Sarah H.; Kummrow, Fabio; Claxton, Larry D. (CETESB-Cia de Tecnologia de Saneamento Ambiental, Sao Paulo 05459-900, Brazil). Food and Chemical Toxicology, 43(1), 49-56 (English) 2005 Elsevier B.Chemicals with cas numbers 9037-41-6 and 56548-64-2 also play role.V. CODEN: FCTOD7. ISSN: 0278-6915. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Textile dyes can enter the water ecosystem through wastewater discharges potentially exposing humans through the consumption of water and food. The com. disperse dye product CI Disperse Blue 291 contg. the aminoazobenzene 2-[(2-bromo-4,6-dinitrophenyl)azo]-5-(diethylamino)-4-methoxyacetanili de (CAS registry no. 56548-64-2) was tested for mutagenic activity in the Salmonella assay. We used strains with different levels of nitroreductase and O-acetyltransferase (i.e., TA98DNP6, YG1024, and YG1041) that are relevant enzymes in the activation of nitrocompounds by the intestinal microflora. The com. product tested also was mutagenic for TA1537, TA1538, TA98 and TA100. Presence of the pKM101 plasmid and the addn. of S9 enhanced the mutagenic response. Specialized strains showed that both nitroreductase and O-acetyltransferase are important in activation of the product. The highest potency obtained was 240 revertants per mg for YG1041 in the presence of S9. Besides being able to cause frameshift mutations (hisd3052), the dye was able to cause all types of base pair substitution with a preference for TA to AT; CG to TA and CG to AT changes. With these results clearly showing that the bacterial nitroreductase and O-acetyltransferase metabolites of this compd. are mutagenic, there is a need to test this dye using in vivo systems to verify possible adverse effects of this product in mammalian tissues. .