Detail of "566-75-6"

Reference

Estrogen 2-, 4-, 6- or 16-hydroxylation by human follicles shown by gas chromatography-mass spectrometry associated with stable isotope dilution

Estrogen 2-, 4-, 6- or 16-hydroxylation by human follicles shown by gas chromatography-mass spectrometry associated with stable isotope dilution. Dehennin, L.; Blacker, C.; Reiffsteck, A.; Scholler, R. (Fond. Rech. Hormonol., Paris 75014, Fr.). J. Steroid Biochem., 20(1), 465-71 (English) 1984. CODEN: JSTBBK. ISSN: 0022-4731. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 13 Human follicular fluid, obtained by aspiration of human Graafian follicles in cycles stimulated by clomiphene and gonadotropins, was analyzed for estrogen content. The identified estrogens and their mean concns. (ng/mL) are: 2-hydroxy-estrone [362-06-1] (0.14), 4-hydroxy-estrone [3131-23-5] (0.12), 2-hydroxy-estradiol [362-05-0] (0.36), 4-hydroxy-estradiol [5976-61-4] (0.34), 6a-hydroxy-estradiol [1229-24-9] (13.Several substances with their cas registry numbers 57-91-0 and 1229-24-9 may be metioned in this study.2), 6b-hydroxy-estradiol [3583-03-7] (6.40), 2-methoxy-estrone [362-08-3] (0.83), 2-methoxy-estradiol [362-07-2] (10.5), 16-oxo-estradiol [566-75-6] (0.41), estriol [50-27-1] (10.2), estradiol-17b [50-28-2] (1365), estradiol-17a [57-91-0] (1.91), and estrone [53-16-7] (211). Metab. of estradiol by 6-hydroxylation seems to be predominant in the human ovary. The other data suggest that 2-hydroxylation, with subsequent O-methylation, and 16-hydroxylation may be by equiv. pathways, since the sum of the 2-methoxy-estrone and 2-methoxyestradiol concns. is rather similar to the estriol concn. Hence, the latter 3 compds. and the 6-hydroxy-estradiols may be end-products of follicular estrogen metab. Catechol estrogen formation by 2-hydroxylation and 4-hydroxylation is of equal importance in the ovary. These results confirm the presence in the human follicle of various competing estrogen hydroxylases and catechol-O-methyltransferase. .