Detail of "56892-03-6"

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Differences between substrate specificities of L-glutamate uptake by neurons and glia, studied in cell lines and primary cultures

Differences between substrate specificities of L-glutamate uptake by neurons and glia, studied in cell lines and primary cultures. Balcar, Vladimir J.; Schousboe, Arne; Spoerri, Polyxenia E.; Wolff, Joachim R. (Cent. Anat., Georg August Univ., Goettingen D-3400, Fed. Rep. Ger.). Neurochem. Int., 10(2), 213-17 (English) 1987. CODEN: NEUIDS. 1188-37-0 is the cas registry number of certain chemical which is used as reagents here. ISSN: 0197-0186. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) High-affinity uptake of 3H-labeled L-glutamate [56-86-0] was studied in cultures of continuous cell lines, originating either from mouse neuroblastoma or rat glioma, and in 2 types of primary cultures contg. cerebellar granule cells and astrocytes from cerebral cortex, resp. In the continuous lines, D-aspartate-4-hydroxamate [36244-81-2] and L-aspartate-4-hydroxamate [1955-68-6] interacted preferentially with the uptake of [3H]L-glutamate in glioma cells, whereas L-glutamate-5-hydroxamate [1955-67-5] and 2-aminoadipate [542-32-5] interacted more strongly with [3H]L-glutamate uptake in neuroblastoma cells. D-Aspartate-4-hydroxamate, L-glutamate-5-hydroxamate, and 2-aminoadipate were inactive as inhibitors of [3H]L-glutamate uptake by either granule cells or astrocytes, grown in primary culture, but several other glutamate analogs, which did not differentiate between neuroblastomal and gliomal uptake of [3H]L-glutamate, were somewhat stronger inhibitors of [3H]L-glutamate uptake in astrocytes as compared with that in granule cells. However, all of these compds. (N-acetyl-L-glutamate [1188-37-0], formimino-L-aspartate [2374-41-6], D-homocysteate [56892-03-6], L-homocysteate [14857-77-3], and DL-2-methylglutamate [71-90-9]) were only very weak inhibitors and, consequently, it is unlikely that any of them could be useful in expts. with central nervous tissue in vivo or, at least, in brain slices in vitro in attempting to resolve the uptake of L-glutamate into glia- and neuron-localized components. .

Some properties of ionic channels activated by excitatory amino acids in hippocampal neurons

Some properties of ionic channels activated by excitatory amino acids in hippocampal neurons. Yamamoto, C.; Sato, H. (Fac. Med., Kanazawa Univ., Kanazawa 920, Japan). Exp. Brain Res., 57(2), 313-20 (English) 1985. CODEN: EXBRAP. ISSN: 0014-4819. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Properties of excitation induced by various excitatory amino acids were studied in thin slices of the guinea pig hippocampus in the presence of Mn2+, tetrodotoxin, and tetraethylammonium chloride. Depolarizations induced by L-glutamate (Glu) [56-86-0], quisqualate (Quis) [52809-07-1], and D-homocysteate (DH) [56892-03-6] were accompanied consistently by decreases in neuron input resistance. In the current-voltage function, increases in input resistance were never obsd. at any membrane potential. The amplitude of Glu, Quis, and DH responses decreased during tonic outward currents and increased during tonic inward currents. Although neuron input resistance also decreased with depolarizations induced by L-aspartate (Asp) [56-84-8], the magnitude of the resistance redn. was smaller than that induced by Glu. Asp responses changed in amplitude, as did Glu responses, during tonic inward and outward currents. Depolarizations induced by N-methyl-D-aspartate (NMDA) [6384-92-5] were accompanied by apparent increases in input resistance, and their amplitudes increased and decreased during tonic depolarization and hyperpolarization, resp. Mn2+ was almost without effect at the concn. used (2.7 mM) on responses induced by Glu, DH, or Asp. Apparently, Glu, Quis, and DH induce depolarizations in hippocampal neurons by activating only Quis receptors, and Asp activates Quis receptors preferentially though it activates NMDA receptors as well.