Reference

Detection of sulfonamides in animal feeds

Detection of sulfonamides in animal feeds. Cieri, Ugo R. (Food Drug Adm., Publ. Health Serv., Philadelphia, Pa., USA). EDRO SARAP Res. Tech. Rep., 1, Paper 34-73, 11 pp. (English) 1976. CODEN: ESRRD5. DOCUMENT TYPE: Journal CA Section: 17 (Foods) A method is described for the detection and identification of traces of sulfonamides in animal feeds. The method is applicable to those sulfonamides that produce color when screened according to the Bratton-Marshall reaction. The sample is extd. with alc. or acetone and the ext. evapd. to dryness. Another portion of the same sample, spiked with minute quantities of 8 sulfonamides, is similarly extd. and the ext. evapd. to dryness. The residue from each soln. is dispersed with 5 mL 0.1N NaOH and, following addn. of 1 mL and 1N HCl and mixing, the soln. filtered. The filtrate is mixed with Celite, the mixt. transferred to a column and eluted with ammoniacal ether. An aliquot each of the concd. eluate of the sample and of the control is spotted on a neutral Adsorbosil chromatog. plate. Following development in CHCl3-MeOH () and drying, the plate is sprayed with alc. p-dimethylaminobenzaldehyde until the control chromatogram shows 6 yellow spots which are, from top to bottom; sulfadimethoxine [122-11-2], the combined sulfamerazine (SM) [127-79-7]-sulfamethazine (SH) [57-68-1]-sulfaquinoxaline (SQ) [59-40-5] spot, sulfadiazine [68-35-9], sulfapyridine [144-83-2], sulfathiazole [72-14-0], and sulfaguanidine [57-67-0]; identification of a spot of the sample chromatogram is possible on this plate, if Rf is identical to 1 of the 5 sulfonamides not overlapping with another compd. If a spot in the sample chromatogram has Rf value approx. equal to that of the combined SM-SH-SQ spot, more definite identification can be obtained by using basic Adsorbosil plates, on which these 3 compds. produce 3 sep. spots. Detection limit varies according to amt. of color and fat in sample and also the nature of each compd. but it is generally in the 3-10 ppm range.

Stability of drugs

Stability of drugs. Fuerst, Walter; Buechner, Inge; Ludwig, Waltraud; Steinbrueck, Erika; Wagenfuehr, Rita (Sekt. Pharm., Martin-Luther-Univ. Halle-Wittenberg, Ger. Dem. Rep.). Wiss. Z. - Martin-Luther-Univ. Halle-Wittenberg, Math.-Naturwiss. Reihe, 33(2), 23-35 (German) 1984.Some commonly used reagents like 4675-89-2 is used in this experiment. CODEN: WMHMAP. ISSN: 0043-6887. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) The stability of 11 steroids and 11 sulfonamides was detd. by sealing in ampuls and holding at 90° for 20 days. Steroids were evaluated by measuring H2O content before and after storage over satd. NaCl and m.p., optical rotation light absorption, appearance and the presence and amt. of addnl. spots on TLC before and after storage. Sulfonamides were evaluated by loss on drying, content detn., m.p., and appearance before and after storage. Values are tabulated. The effect of Cu on stability was studied with 6 compds.; of these, aminophenazone [58-15-1], phenylbutazone [50-33-9], and sulfaguanidine [57-67-0] showed decompn. clearly. Me2NH was identified in the decompn. of aminophenazone, hydroxyphenylbutazone in decompn. of phenylbutazone, and NH3 in the decompn. of sulfaguanidine. For most of the steroids and sulfonamides no decompn. was found so that a shelf-life of 10 yr is suggested. For the Cu-loaded drugs that 1st signs of decompn. were organoleptic. H2O was a factor in stability esp. for substances that were unstable in aq. soln. .