Detail of "5813-86-5"

Reference

Potential of cell killing by inhibitors of poly(adenosine diphosphate-ribose) synthesis in bleomycin-treated Chinese hamster ovary cells

Potential of cell killing by inhibitors of poly(adenosine diphosphate-ribose) synthesis in bleomycin-treated Chinese hamster ovary cells. Huet, J.; Laval, F. (Groupe "Radiochim. ADN", Inst. Gustave-Roussy, Villejuif 94805, Fr.). Cancer Res., 45(3), 987-91 (English) 1985. CODEN: CNREA8. ISSN: 0008-5472. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Bleomycin [11056-06-7]-treated Chinese hamster ovary cells synthesize poly(ADP-ribose) [26656-46-2] in a reaction which is dose- and time-dependent. Treatment with 2 poly(ADP-ribose) synthesis inhibitors (3-aminobenzamide [3544-24-9] and 3-methoxybenzamide [5813-86-5]) slows down the restoration of DNA structure in bleomycin-treated cells, as shown by nucleoid sedimentation. When added in the culture medium, these inhibitors increase the cell sensitivity towards bleomycin, in the case of both exponentially growing and stationary-phase cells. In control expts., plateau-phase cells treated with bleomycin can recover by repairing efficiently the potentially lethal damage; this type of repair is mostly suppressed in the presence of the poly(ADP-ribose) synthesis inhibitors.

Effect of nicotinamide analogs on recovery from DNA damage in C3H10T1/2 cells

Effect of nicotinamide analogs on recovery from DNA damage in C3H10T1/2 cells. Jacobson, Elaine L.; Smith, Janice Yoder; Mingmuang, Mingkwan; Meadows, Rene; Sims, James L.; Jacobson, Myron K. (Dep. Biol., Texas Woman's Univ., Denton, TX 70624, USA). Cancer Res., 44(6), 2485-92 (English) 1984. CODEN: CNREA8. ISSN: 0008-5472. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The effects of nicotinamide analogs on cellular recovery following N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) [70-25-7] treatment were characterized in the transformable cell line, C3H10T1/2. The recovery of cell division potential was measured under conditions which allow simultaneous quantification of intracellular levels of poly(ADP ribose) [26656-46-2], NAD [53-84-9], and rates of RNA, DNA, and protein synthesis. 3-Methoxybenzamide (MBA) [5813-86-5], 3-aminobenzamide [3544-24-9], and benzamide [55-21-0], which are effective inhibitors of ADP ribosyltransferase [58319-92-9], blocked recovery of cell division following treatment with 34 mM MNNG, while the noninhibitors, 3-methoxybenzoate [586-38-9] and benzoate [65-85-0], had no effect. In the presence of MBA, cells progressively lost the ability to resume cell division during the first 24 to 36 h following DNA damage. The intracellular levels of poly(ADP ribose) increased approx. 7-fold within 20 min following MNNG treatment, and 1 mM MBA inhibited this increase by ~82%. In the presence of MBA, a dramatic decrease in the rate of DNA synthesis occurred approx. 16 h after MNNG treatment, while RNA and protein synthesis continued at rates similar to those in cells treated with MNNG alone.