Detail of "58626-38-3"

Reference

Enzyme immunoassay of the antibiotic viomycin

Enzyme immunoassay of the antibiotic viomycin. Fujitake, Takafumi; Kitagawa, Tsunehiro; Aikawa, Tadaomi (Fac. Pharm. Sci., Nagasaki Univ., Nagasaki, Japan). Rinsho Kagaku Shimpojumu, 16, 18-22 (Japanese) 1976. CODEN: RKASDA. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Viomycin (I) [32988-50-4] (6 .mu.mol) was treated with N-(m-maleimidobenzyloxy)succinimide (MBS) [58626-38-3] and .beta.-galactosidase [9031-11-2] to obtain a I-MBS-.beta.-galactosidase conjugate. The conjugate was treated with various concns. of antiserum to I in the presence or in the absence of I, and the enzyme activities of resulting ppts. were measured. The competitive binding test between the conjugate and I to the I-antiserum indicated that I is detected at concns. ranging between 100 pg to 4 ng.

Chemical methods for the modification of liposomes with proteins or antibodies

Chemical methods for the modification of liposomes with proteins or antibodies. Hashimoto, Yoshiyuki; Endoh, Hiroo; Sugawara, Minoru (Dep. Hyg. Chem., Tohoku Univ., Sendai, Japan). Liposome Technol., Volume 3, 41-9. Edited by: Gregoriadis, Gregory. CRC: Boca Raton, Fla. (English) 1984. CODEN: 52BBAJ. DOCUMENT TYPE: Conference CA Section: 63 (Pharmaceuticals) Section cross-reference(s): 6 Tolylene 2,4-diisocyanate (TDIC) [584-84-9], a crosslinking reagent binding 2 NH2 groups, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI) [1892-57-5], binding NH2 and CO2H groups, and m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) [58626-38-3] crosslinking NH2 and SH groups, were used as coupling agents to prep. protein-or antibody-modified liposomes. TDIC was used to prepd. dansyl-bovine serum albumin-modified liposomes, EDCI for anti-SRBC IgG modified liposome, and MBS reactions with phospholipids. Assay of liposome-surface protein, targeting of antibody-modified liposomes to antigenic cells, and application of antibody-modified liposomes are discussed.