Detail of "590-97-6"

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Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging

Some chemicals with cas registry numbers like 825628-77-1 and 590-97-6 are also used. Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging. Komatsu, Hirokazu; Iwasawa, Naoko; Citterio, Daniel; Suzuki, Yoshio; Kubota, Takeshi; Tokuno, Kentaro; Kitamura, Yoshiichiro; Oka, Kotaro; Suzuki, Koji (Departments of Applied Chemistry and of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Yokohama, Kanagawa 223-8522, Japan). Journal of the American Chemical Society, 126(50), 16353-16360 (English) 2004 American Chemical Society. CODEN: JACSAT. ISSN: 0002-7863. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) The role of intracellular magnesium ions is of high interest in the fields of pharmacol. and cellular biol. To accomplish the dynamic and three-dimensional imaging of intracellular Mg2+, there is a strong desire for the development of optimized Mg2+ fluorescent probes. In this paper we describe the design, synthesis, and cellular application of the three novel Mg2+ fluorescent probes KMG-101, -103, and -104. The compds. of this series feature a charged b-diketone as a binding site specific for Mg2+ and a fluorescein residue as the fluorophore that can be excited with an Ar+ laser such as is widely used in confocal scanning microscopy. This mol. design leads to an intensive off-on-type fluorescent response toward Mg2+ ions. The two fluorescent probes KMG-103 and -104 showed suitable dissocn. consts. (Kd,Mg2+ = 2 mM) and nearly a 10-fold fluorescence enhancement over the intracellular magnesium ion concn. range (0.1-6 mM), allowing high-contrast, sensitive, and selective Mg2+ measurements. For intracellular applications, the membrane-permeable probe KMG-104AM was synthesized and successfully incorporated into PC12 cells. Upon application of the mitochondria uncoupler FCCP to the probe-incorporated cells, the resulting increase in the free magnesium ion concn. could be followed over time. By using a confocal microscope, the intracellular 3D magnesium ion concn. distributions were satisfactorily obsd. .

Preparation of carbocyclic nucleotide acyloxyalkyl esters as membrane-permeant second messengers

Preparation of carbocyclic nucleotide acyloxyalkyl esters as membrane-permeant second messengers. Tsien, Roger Y.; Schultz, Carsten; Li, Wenhong (Regents of the University of California, USA). PCT Int. Appl. WO 9640695 A1 19 Dec 1996, 41 pp. DESIGNATED STATES: W: AU, CA, JP; RW: AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE. (English). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: C07F009-02. ICS: A61K031-025; A61K031-66. APPLICATION: WO 1996-US9923 7 Jun 1996. PRIORITY: US 1995-475758 7 Jun 1995. DOCUMENT TYPE: Patent CA Section: 33 (Carbohydrates) Section cross-reference(s): 6, 7 Cyclitol acyloxyalkyl phosphate esters second messengers which are capable of permeating cell membranes. Once inside the cell, the ester derivs. undergo enzymic conversion to the biol. active form of the second messenger. 590-97-6 and 51116-00-8 which are cas registry numbers of substances are two of reagents here. The carbocyclic nucleotide acyloxyalkyl esters, e.g., N2,O2'-dibutyryl guanosine monophosphate aacetoxymethyl ester was prepd. as activator of intracellular protein kinase A. .