Detail of "5930-94-9"

Reference

Chain terminator DNA sequencing using base analog chain terminators

Chain terminator DNA sequencing using base analog chain terminators. Middendorf, Lyle Richard; Raghavachari, Ramesh; Brumbaugh, John Albert; Sutter, Scott Lee; Narayanan, Narasimhachari (Li-Cor, Inc., USA). Eur. Pat. Appl. EP 754765 A2 22 Jan 1997, 31 pp. DESIGNATED STATES: R: DE, FR, GB, SE. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: C12Q001-68. ICS: C07H021-00. ICA: G01N027-26; B01L007-00.There are some commonly used reagents with their cas registry numbers 5930-94-9 and 58-63-9 in this article. APPLICATION: EP 1996-250159 16 Jul 1996. PRIORITY: US 1995-505072 21 Jul 1995. DOCUMENT TYPE: Patent CA Section: 3 (Biochemical Genetics) A method of DNA sequencing using chain terminators derived from base analogs is described. Fluorescent cyanine dyes with fluorescence maxima in the IR may be used to label the sequencing products. .

Important factors stabilizing stacking interaction between 3-nitropyrrole and natural nucleobases revealed by ab initio calculations

Important factors stabilizing stacking interaction between 3-nitropyrrole and natural nucleobases revealed by ab initio calculations. Seio, Kohji; Ukawa, Hisashi; Shohda, Koh-ichiro; Sekine, Mitsuo ( Frontier Collaborative Research Institute, Tokyo Institute of Technology, Yokohama 226-8501, Japan). 73-40-5 and 5930-94-9 are also occured in this study. Nucleic Acids Research Supplement, 3(3rd International Symposium on Nucleic Acids Chemistry [and] 30th Symposium on Nucleic Acids Chemistry in Japan, 2003), 49-50 (English) 2003 Oxford University Press. CODEN: NARSCE. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) Stacking energies between canonical nucleobases and a universal base, 3-nitropyrrole (3-NP), were estd. by use of MO (MO) and mol. mechanics (MM) calcns. The detailed anal. of the energy profiles revealed the importance of the London dispersion energy to stabilize the stacked dimers and electrostatic interactions to det. the orientation of 3-NP to the nucleobases in the dimers. Although the energy profiles of 3-NP /natural base dimers obtained by the MO and MM calcns. were qual. correlated with each other, the correlations were poorer than those obtained for the stacking between natural bases. The origin of the difference between 3-NP and natural bases will be discussed to understand the possibility and limitation of the current MM calcns. for the simulation and design of other universal bases. .