Detail of "5939-37-7"

CAS Number:
5939-37-7
Name:
L-Glutamic acid,N-[4-[[(2,4-diamino-7,8-dihydro-7-oxo-6-pteridinyl)methyl]methylamino]benzoyl]-
Molecular Structure:
Formula:
C20H22 N8 O6
Molecular Weight:
470.50
Synonyms:
Glutamicacid, N-[p-[[(2,4-diamino-7-hydroxy-6-pteridinyl)methyl]methylamino]benzoyl]-(7CI); Glutamic acid,N-[p-[[(2,4-diamino-7-hydroxy-6-pteridinyl)methyl]methylamino]benzoyl]-, L-(+)-(8CI); L-Glutamic acid,N-[4-[[(2,4-diamino-1,7-dihydro-7-oxo-6-pteridinyl)methyl]methylamino]benzoyl]-(9CI); 7-Hydroxymethotrexate; NSC 380962
Melting Point:
230ºC dec.
Safety:
A poison by intravenous route. When heated to decomposition it emits toxic vapors of NO_x. Details

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Rapid determination of methotrexate and 7-hydroxymethotrexate in serum and cerebrospinal fluid by radial compression liquid chromatography: Rapid determination of methotrexate and 7-hydroxymethotrexate in serum and cerebrospinal fluid by radial compression liquid chromatography. El-Yazigi, Adnan; Martin, Cazemiro R. (Res. Cent., King Faisal Spec. Hosp., Riyadh 11211, Saudi Arabia). J. Liq. Chromatogr., 7(8), 1579-91 (English) 1984. CODEN: JLCHD8. ISSN: 0148-3919. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) A rapid, specific, and sensitive radial compression reverse phase liq. chromatog. method for the anal. of methotrexate [59-05-2] and 7-hydroxymethotrexate [5939-37-7] in serum and cerebrospinal fluid is reported. A mobile phase consisting of MeCN-MeOH-phosphate (8:) at 6 mL/min flow rate was employed. The U.V. detector was set at 317 nm, and folic acid was used as an internal std. Rapid extn. of methotrexate and 7-hdyroxymethotrexate was performed using Sep-Pak cartridges with high extn. efficiency for both compds. Patient serum and cerebrospinal fluid samples were analyzed by the described method and the concns. of methotrexate were compared to those obtained by an enzyme immunoassay. No interference from other metabolites or anticancer drugs in the chromatog. assay was obsd.

Determination of methotrexate in serum by a column back-flush method: Determination of methotrexate in serum by a column back-flush method. Ahr, H. J.; Becker, D.; Keller, H. E. (Zentrallab., Med. Universitaetsklin., Homburg/Saar D-6650, Fed. Rep. Ger.). Fresenius' Z. Anal. Chem., 318(3-4), 251-2 (German) 1984. CODEN: ZACFAU. ISSN: 0016-1152. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Methotrexate (I) [59-05-2] and its metabolite 7-hydroxymethotrexate [5939-37-7] were detd. in patient serum by a backflush-high-performance liq. chromatog. method. The backflush-HPLC system consisted of a Rheodyne 7125 injector, 6-way ventil Rheodyne 7010, and 2 pumps M6000A and M45. For UV detection at l = 303 nm, a Perkin-Elmer-LC-75 detector was used. Blood samples contg. I were injected into a precolumn packed with Corasil C 18, and were washed with an eluant (5 mM PIC A, pH 7.5) which resulted in removal of the blood protein matrix from I and complete retention of the latter on the precolumn. Chromatog. of I and its metabolite was achieved with m Bondapak (C18, 30 cm, 4 mm) and an eluent consisting of 5 mM PIC A:MeOH (). With this method, the recovery is 100%, and the day-to-day precision is 3.5-5.4%. This method may be used for the detn. of I in the concn. range of 0.1-2000 mmol/L.