Reference

Glucose-specific poly(allylamine) hydrogels - A reassessment

All Rights Reserved. Glucose-specific poly(allylamine) hydrogels - A reassessment. Fazal, Furqan M.; Hansen, David E. (Department of Chemistry, Amherst College, Amherst, MA 01002, USA). Bioorganic & Medicinal Chemistry Letters, 17(1), 235-238 (English) 2007 Elsevier Ltd. CODEN: BMCLE8. ISSN: 0960-894X. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 63 Polymer hydrogels synthesized by crosslinking poly(allylamine hydrochloride) with (±)-epichlorohydrin in the presence of D-glucose-6-phosphate monobarium salt do not show imprinting on the mol. level. A series of hydrogels was prepd. using the following five templates: D-glucose-6-phosphate monobarium salt, D-glucose, L-glucose, barium hydrogen phosphate (BaHPO4), and D-gluconamide; a hydrogel was also prepd. in the absence of a template. For all six hydrogels, batch binding studies were conducted with D-glucose, L-glucose, D-fructose, and D-gluconamide.Several substances with their cas registry numbers 5996-16-7 and 57-48-7 may be metioned in this study. The extent of analyte sugar binding was detd. using 1H NMR. Each hydrogel shows approx. the same relative binding affinity for the different sugar derivs., and none displays selectivity for either glucose enantiomer. The results of the binding studies correlate with the octanol-water partition coeffs. of the sugars, indicative that differential solubilities in the bulk polymer account for the binding affinities obsd. Thus, in contrast to templated hydrogels prepd. using methacrylate- or acrylamide-based reagents, true imprinting does not occur in this novel, crosslinked-poly(allylamine hydrochloride) system. .