Detail of "6012-97-1"

Reference

Sequence similarity and cross-reactivity of a Diels-Alder catalyst and an anti-progesterone antibody

Sequence similarity and cross-reactivity of a Diels-Alder catalyst and an anti-progesterone antibody. Haynes, Matthew R.; Lenz, Martin; Taussig, Michael J.; Wilson, Ian A.; Hilvert, Donald (Dep. Molecular Biol. Chem., Scripps Res. Inst., La Jolla, CA 92037, USA). Israel Journal of Chemistry, 36(2), 151-159 (English) 1996 Laser Pages Publishing. CODEN: ISJCAT. ISSN: 0021-2148. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) Antibody 1E9 catalyzes the bimol. Diels-Alder reaction between tetrachlorothiophene dioxide and N-ethylmaleimide with high catalytic efficiency. The genes encoding the heavy and light chains were cloned, sequenced, and expressed as a mouse/human chimera in a murine hybridoma. Comparison with the closely related and structurally characterized anti-progesterone antibody DB3 has permitted identification of the residues that are likely to line the binding cavity. In addn. to confirming the expectation of a highly hydrophobic active site, modeling results suggest that shape complementarity, achieved mainly through van der Waals rather than hydrogen bonding or electrostatic interactions, is likely to be the dominant factor in achieving effective preorganization of the substates for catalysis. These conclusions can now be investigated through targeted mutagenesis of the putative active site residues exploiting the mammalian expression system.Except for chemicals metioned above, 6012-97-1 and 186985-04-6 are also used. .

The action of nitrenes on halothiophenes: formation of thiophene S,N-ylides

The action of nitrenes on halothiophenes: formation of thiophene S,N-ylides. Meth-Cohn, Otto; Van Vuuren, Gerda (Natl. Chem. Res. Lab., Counc. Sci. Ind. Res., Pretoria 0001, S. Afr.). J. Chem. Soc., Chem. Commun.There are some reagents like 6012-97-1 is used in this study., (3), 190-1 (English) 1984. CODEN: JCCCAT. ISSN: 0022-4936. DOCUMENT TYPE: Journal CA Section: 27 (Heterocyclic Compounds (One Hetero Atom)) Section cross-reference(s): 22, 28 Decompn. of N3CO2R (R = Et, Ph) and N3SO2C6H4Me-p in tetrachlorothiophene at 130-150° gave the S,N-ylides I (R = CO2Et, CO2Ph, SO2C6H4Me-p) in 44, 23, and 24% yield, resp. In contrast, analogous reaction of 2,5-dichloro- and -dibromo- and tetrabromothiophenes with N3CO2Ph gave products arising from a-attack followed by rearrangement; 83% benzoxazalone II was obtained from tetrabromothiophene. .