Detail of "60311-02-6"

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A two-parameter flow cytometry protocol for the detection and characterization of the clastogenic, cytostatic and cytotoxic activities of chemicals

A two-parameter flow cytometry protocol for the detection and characterization of the clastogenic, cytostatic and cytotoxic activities of chemicals. Maier, P.; Schawalder, H. P. (Inst. Toxicol., Swiss Fed. Inst. Technol., Schwerzenbach CH-8603, Switz.). Mutat. Res., 164(6), 369-79 (English) 1986. CODEN: MUREAV. ISSN: 0027-5107. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Cultured, freshly-isolated rat fibroblasts were exposed in vitro to vincristine sulfate (VC) [2068-78-2], amethopterin (AM) [59-05-2], bleomycin (BL) [11056-06-7], benomyl (BE) [17804-35-2], and practolol (PR) [6673-35-4]. Cells treated for 5 h were subjected 24 h later to a 2-parameter (DNA/protein) flow cytometry anal. The fluorochromes used were sulforhodamine 101 [60311-02-6] and DAPI [47165-04-8]. From DNA and protein histograms, alterations in cell-cycle kinetics, variations in the amt. of DNA in individual G1-phase cells, and the enhancement of or increased variation in the protein content of the exposed cells were detd. Each of the 5 chems. induced a specific dose-dependent pattern of changes in the DNA and protein histograms. DNA dispersion was enhanced with VC, AM, BL, and BE but not with PR. The cell cycle was blocked in the G2-phase with VC, at early S-phase with AM and depending on the dose, at the G1- or G2-phase with BL or at the S-phase or G2-phase with BE. PR inhibited cells slightly in the S-phase at the highest exposure level. Protein anal. allows cytotoxic activity (loss of proteins) or induced unbalanced growth (protein accumulation) of test compds. to be recognized. Thus, the proposed 2-parameter DNA/protein anal. by flow cytometry is a suitable method for prospective testing of chems. for their induction of structural or numerical chromosome aberrations. Simultaneously, a broad range of cytotoxic, cytostatic, and cell-cycle perturbing activities of the test agents can be recognized.

Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques

All Rights Reserved. Interfacial behavior of sulforhodamine 101 at the polarized water/1,2-dichloroethane interface studied by spectroelectrochemical techniques. Nagatani, Hirohisa; Suzuki, Shingo; Fermin, David J.; Girault, Hubert H.; Nakatani, Kiyoharu (Department of Applied Chemistry, Faculty of Engineering, Nagasaki University, Bunkyo 852-8521, Japan). Analytical and Bioanalytical Chemistry, 386(3), 633-638 (English) 2006 Springer. CODEN: ABCNBP. ISSN: 1618-2642. DOCUMENT TYPE: Journal CA Section: 72 (Electrochemistry) Section cross-reference(s): 66, 73 The transfer mechanism of an amphoteric rhodamine, sulforhodamine 101 (SR101), across the polarized H2O/1,2-dichloroethane (DCE) interface was studied using cyclic voltammetry, differential voltfluorometry and potential-modulated fluorescence (PMF) spectroscopy. The voltammetric response for the ion transfer of SR101 monoanion from H2O to DCE was obsd. as the diffusion-controlled transfer process. An unusual voltammetric response was found at 0.15 V more neg. than the formal transfer potential of SR101- (DWOf0') in the cyclic voltammogram and voltfluorogram. The frequency dependence of the PMF responses confirmed the presence of the adsorption processes at neg. potentials. A further transient adsorption step was uncovered at DWOf0'. 60311-02-6 and 7791-03-9 are just another two chemicals used in this study. The interfacial mechanism of SR101 is discussed by comparing the results obtained from each technique. .