Reference

Actions of local anesthetics on cultured human cells

Actions of local anesthetics on cultured human cells. Dawson, M.; Mustafa, A.In this study， 136-47-0 and 24561-10-2 are also used. F. (Dep. Pharm. Technol., Univ. Strathclyde, Glasgow, Scot.). J. Pharm. Pharmacol., 28, Suppl. 9P (English) 1976. CODEN: JPPMAB. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) During incubation of human cell cultures (H. Ep. 2 and Flow 2000) with local anesthetics at normal usage concns., the effect on cell structure and function showed that procaine-HCl [51-05-8], lignocaine-HCl [73-78-9], and piperocaine-HCl [24561-10-2] were nontoxic, but amylocaine-HCl [532-59-2], amethocaine-HCl [136-47-0], and cinchocaine-HCl [61-12-1] were progressively toxic. .

Effects of local anesthetics on intracellular fusion processes

Effects of local anesthetics on intracellular fusion processes. Enhancement of concanavalin A-induced macrophage vacuolation. Raz, Avraham; Goldman, Rachel (Dep. Membrane Res., Weizmann Inst. Sci., Rehovot, Israel). Biochim. Biophys. Acta, 455(1), 226-40 (English) 1976. CODEN: BBACAQ. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) The extensive vacuolation elicited in mouse peritoneal macrophages in response to concanavalin A [11028-71-0] was markedly enhanced by a simultaneous exposure to anesthetics. The potency of enhancing vacuolation increased within the series of normal alcs. with chain length C10 > C8 > C7 > C6. Lidocaine-HCl [73-78-9] and procaine-HCl [51-05-8] were by far more effective than tetracaine-HCl [136-47-0] and dibucaine-HCl [61-12-1]. The latter 2 induced extensive cell shrinkage at concns. at which the first 2 exhibited optimum enhancing capacity. Of the tested compds. chlorpromazine-HCl [50-53-3] had the highest membrane/buffer partition coeff. and it exhibited its optimum enhancing effect on concanavalin A-induced macrophage vacuolation at the lowest concn. The binding of [3H] concanavalin A as well as its internalization by macrophages incubated with the lectin for 15, 45 and 90 min were not affected significantly in the presence of decanol [112-30-1], procaine, or chlorpromazine at concns. of max. effect on vacuolation. Thus enhancement of vacuolation does not stem from an increase in the rate or extent of concanavalin A interiorization.There are some commonly used reagents with their cas registry numbers 73-78-9 and 112-30-1 in this article. The rate at which vacuoles were generated was however markedly increased in the presence of chlorpromazine and the resulting vacuoles were of a larger diam. At 2-5 times the concn. required for inhibition of max. enhancing effect, the drugs lead to extensive macrophage shrinkage and to depletion of intracellular ATP [56-65-5]. Phagocytosis of heat-killed yeast cells was reduced by tertiary amine anesthetics at concns. optimal for enhancement of concanavalin A-induced vacuolation. Enhanced intracellular fusion of concanavalin A-bearing pinosomes to form vacuoles is discussed in terms of current ideas on factors affecting membrane fusion and the effects of anesthetics on membrane organization of lipids, intramembraneous particles, glycoprotein receptors, and the possible control by cytoskeletal elements. .