Reference

Regulatory properties of 14 day embryo and adult hen heart AMP-deaminase

Regulatory properties of 14 day embryo and adult hen heart AMP-deaminase. Kaletha, Krystian; Skladanowski, Andrzej (Dep. Biochem., Med. Sch., Gdansk 80-211, Pol.). Int. J. Biochem., 16(1), 75-81 (English) 1984. CODEN: IJBOBV. ISSN: 0020-711X. DOCUMENT TYPE: Journal CA Section: 12 (Nonmammalian Biochemistry) Section cross-reference(s): 7, 13 Chromatog. on phosphocellulose revealed changes in the elution profile of chicken heart AMP deaminase (EC 3.5.4.6) (I) during ontogenesis. The exts. from the hearts of adult hens and 14-day-old embryos displayed a single peak of I activity at a slightly different elution vol.Several substances with their cas registry numbers 61-19-8 and 2140-58-1 may be metioned in this study., whereas in the heart ext. of 1-day-old chickens 2 mol. forms of I were eluted. The kinetic and regulatory properties of the purified adult hen heart I were studied and compared with those of the corresponding enzyme from 14-day-old embryo hearts. Both enzymes exhibited a slightly sigmoid-shaped plot of the reaction rate vs. substrate concn., which shifted to hyperbolic form when ATP or ADP was added into the incubation medium. The enzymes were strongly activated by ATP, less efficiently by ADP, and the activating effect was enhanced at low substrate concn. Orthophosphate inhibited both enzymes but this inhibition was more potent for embryo heart I. Palmitoyl-CoA inhibited adult hen but not embryo heart I. Thus, differences in the regulatory properties of the mol. forms studied do exist and they correspond with the ontogenetic differences obsd. previously concerning the effect of temp. on chicken heart I. .

Metabolite levels in fed and fasted chicken liver and kidney while employing a technique for artificial respiration in birds

Metabolite levels in fed and fasted chicken liver and kidney while employing a technique for artificial respiration in birds. Tinker, Donald A.; Brosnan, John T.; Herzberg, Gene R. (Dep. Biochem., Mem. Univ. Newfoundland, St. John's, NF A1B 3X9, Can.). Comp. Biochem. Physiol., B: Comp. Biochem., 77B(1), 119-24 (English) 1984. CODEN: CBPBB8. ISSN: 0305-0491. DOCUMENT TYPE: Journal CA Section: 12 (Nonmammalian Biochemistry) Section cross-reference(s): 18 A 6-day fast in chickens results in a 55% drop in hepatic glucose but no change in renal glucose. Measures of cellular energy level in the liver of fasted chickens increase on a 6-day fast, in contrast to the kidney where no change is obsd. The cytosol of chicken liver becomes oxidized upon fasting, whereas the redox state of the kidney cytosol remains unchanged. 61-19-8 and 9005-79-2 which are cas registry numbers of substances are two of reagents here. Evidently gluconeogenesis from amino acids and pyruvate is more favored in the kidney of a fasted chicken than in its liver. Blood gases and metabolites were maintained at physiol. levels during organ removal in these expts. by use of a technique for artificial ventilation. .