Reference

Performance characteristics of an ion chromatographic method for the quantitation of citrate and phosphate in pharmaceutical solutions

All Rights Reserved. Performance characteristics of an ion chromatographic method for the quantitation of citrate and phosphate in pharmaceutical solutions. Jenke, Dennis; Sadain, Salma; Nunez, Karen; Byrne, Frances ( Technology Resources Division, Baxter Healthcare Corporation, Round Lake, IL 60073, USA). Journal of Chromatographic Science, 45(1), 50-56 (English) 2007 Preston Publications. CODEN: JCHSBZ. ISSN: 0021-9665. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) The performance of an ion chromatog. method for measuring citrate and phosphate in pharmaceutical solns. is evaluated. Performance characteristics examd. include accuracy, precision, specificity, response linearity, robustness, and the ability to meet system suitability criteria. In general, the method is found to be robust within reasonable deviations from its specified operating conditions. Anal. accuracy is typically 100±3%, and short-term precision is not more than 1.5% relative std. deviation. The instrument response is linear over a range of 50% to 150% of the std. prepn. target concns. (12 mg/L for phosphate and 20 mg/L for citrate), and the results obtained using a single-point std. vs. a calibration curve are essentially equiv. A small anal. bias is obsd. and ascribed to the relative purity of the differing salts, used as raw materials in tested finished products and as ref. stds. in the anal. method. The assay is specific in that no phosphate or citrate peaks are obsd. in a variety of method-related solns.There are some commonly used reagents with their cas registry numbers 7778-77-0 and 6132-04-3 in this article. and matrix blanks (with and without autoclaving). The assay with manual prepn. of the eluents is sensitive to the compn. of the eluent in the sense that the eluent must be effectively degassed and protected from CO2 ingress during use. In order for the assay to perform effectively, extensive system equilibration and conditioning is required. However, a properly conditioned and equilibrated system can be used to test a no. of samples via chromatog. runs that include many (> 50) injections. (c) 2007 Preston Publications. .

Chemical method of breaking cell-loaded sodium alginate/chitosan microcapsules

Chemical method of breaking cell-loaded sodium alginate/chitosan microcapsules. Xue, Wei-Ming; Yu, Wei-Ting; Liu, Xiu-Dong; He, Xin; Wang, Wei; Ma, Xiao-Jun (Lab. Biomed. Mater. Eng., Dalian Inst. Chem. 6132-04-3 and 144-55-8 are also occured in this study. Phys., Chinese Acad. Sci., Dalian 116023, Peop. Rep. China). Gaodeng Xuexiao Huaxue Xuebao, 25(7), 1342-1346 (Chinese) 2004 Gaodeng Jiaoyu Chubanshe. CODEN: KTHPDM. ISSN: 0251-0790. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Section cross-reference(s): 9 A novel chem. method to break and dissolve cell-loaded microcapsules under physiol. condition was established, esp. aiming at alginate/chitosan/alginate(ACA) microcapsules. The microcapsule-broken process was designed to proceed under an approx. physiol. condition for the consideration of maintaining the cytoactive. The microbial cell (Pichia pastoris GS115) and animal cell (L929) were chosen as-the model cells, and the microcapsule-broken soln.(MBS) was basically composed of NaHCO3 and Na3C6H5O7 × 2H8O. The effects of MBS on the breaking of microcapsules and on the cytoactive were thoroughly investigated. Moreover, the mechanism of the breaking of microcapsules was analyzed. It was demonstrated that the microcapsules' could. be broken within 30 s, .the breaking ratio, of the microcapsule was 100%, and the fragments of the microcapsules was dissolved completely. It was also detd. that. the survival rate. of cells was above 85%. Thus, the novel method will make it possible to quant. analyze the growth of cells in microcapsules, to examine the morphol. arid function of cells, to recover the cells and to purify metabolic products, which is always difficult to realize at present. Therefore, the chem. method of breaking and dissolving cell-loaded microcapsules would be the important component of microencapsulation technol. applied to the cell culture and cell transplantation. .