Reference

Comparative efficacies of piperacillin, azlocillin, ticarcillin, aztreonam, and tobramycin against experimental Pseudomonas aeruginosa pneumonia

Comparative efficacies of piperacillin, azlocillin, ticarcillin, aztreonam, and tobramycin against experimental Pseudomonas aeruginosa pneumonia. Schiff, Jill B.; Pennington, James E. (Dep. Med., Brigham and Women's Hosp., Boston, MA 02115, USA). Antimicrob. Agents Chemother., 25(1), 49-52 (English) 1984. CODEN: AMACCQ. ISSN: 0066-4804. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The therapeutic efficacies of the newer b-lactam antibiotics piperacillin [61477-96-1], azlocillin [37091-66-0] and aztreonam [78110-38-0] were compared with the efficacies of ticarcillin and tobramycin in a guinea pig model of exptl. P. aeruginosa pneumonia. For animals challenged with 2 ′ 108 CFU(colony-forming unit), tobramycin treatment resulted in survival rates and intrapulmonary killing of pseudomonads which were significantly greater than those found with any of the b-lactam agents. There were no significant differences noted among the individual b-lactam agents. When animals were challenged with 200-fold-fewer organisms (106 CFU), there was no significant difference between the efficacy of tobramycin and other b-lactams. Thus, tobramycin is particularly valuable in treating more severe P. aeruginosa pneumonia, whereas a no. of different b-lactam agents are of equiv. value in less severe lung infections.

An improved micro-scale liquid-chromatographic assay for piperacillin in plasma and urine

An improved micro-scale liquid-chromatographic assay for piperacillin in plasma and urine. Jung, Donald; Mahajan, Narender K. (Health Sci. Cent., Univ. Illinois, Chicago, IL 60612, USA). Clin. Chem. (Winston-Salem, N. C.), 30(1), 122-4 (English) 1984. CODEN: CLCHAU. ISSN: 0009-9147. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) An accurate, sensitive, and specific liq.-chromatog. method is described for measuring piperacillin (I) [61477-96-1] in plasma and urine. Plasma samples deproteinized with 2 vols. of MeCN contg. 1.2 mg of the internal std., p-nitrobenzene sulfonamide, per L are centrifuged. The clear supernate is evapd. under N, and the residue is reconstituted in 50 mL of the mobile phase (32/68 by vol MeCN/H2O, adjusted to pH 2.5 with 85% phosphoric acid), of which 10 mL is injected onto a reversed-phase (C-18) column. Urine samples are dild. 10-fold with distd. water, an equal vol. of MeCN contg. 3 mg of the internal std. per L is added, and 20 mL is chromatographed. Stability studies indicate that storage conditions are crit. for both plasma and urine. Piperacillin in plasma is stable at -70° for at least 6 wk, but 100% of it is degraded during the same time at -20°. Piperacillin in urine is also stable at -70° for 6 wk, but 20% is degraded during 6 wk at -20°.