Detail of "61926-22-5"

Reference

Determination of rat sperm viability with fluorescence

Determination of rat sperm viability with fluorescence. Kimura, Hitoshi; Kato, Masayuki (Nippon Baio Risaachi Sentaa Kk; Oriental Yeast Co Ltd, Japan). Jpn. 61926-22-5 and 9013-79-0 are also occured in this study. Kokai Tokkyo Koho JP 08332098 A2 17 Dec 1996 Heisei, 4 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: ICM: C12Q001-06. ICS: C12Q001-44; C12Q001-68. APPLICATION: JP 1995-161538 6 Jun 1995. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Fluorescent pigments that permeate viable cell membrane and react with in vivo esterase to get fluorescence, and that permeate only dead or damaged cell membrane are used for detn. of the viability of rat sperm. The method is useful for simultaneous detn. of live and dead sperm and is very efficient. .

Method for reducing the efficiency of primer extension by polymerase enzymes when the 3' end of a primer or growing nucleic acid chain does not hybridize perfectly with the target

Method for reducing the efficiency of primer extension by polymerase enzymes when the 3' end of a primer or growing nucleic acid chain does not hybridize perfectly with the target. Mautner, Martin Eduardo (Argent. ). U.S. Pat. Appl. Publ. US 2006014152 A1 19 Jan 2006,27 pp., Cont.-in-part of U.S. Ser. No.Several reagents with their cas registry numbers 872749-14-9 and 61926-22-5 are used here. 316,745. (English). (United States of America). CODEN: USXXCO. APPLICATION: US 2004-867994 15 Jun 2004. PRIORITY: US 2002-316745 11 Dec 2002. DOCUMENT TYPE: Patent CA Section: 3 (Biochemical Genetics) The invention provides a method for reducing the efficiency of primer extension by polymerase enzymes when the 3' end of a primer does not hybridize perfectly with the target, increasing the selectivity of single nucleotide mutation or gene analyses by suppressing false pos. results. Specifically, the method comprises the steps of obtaining a nucleic acid sample; hybridizing said nucleic acid sample to a primer; subjecting said nucleic acid sample hybridized to a extension reaction by extending a primer with a polymg. enzyme, wherein the reaction extension mixt. medium contains an intercalating agent; and detecting the presence of extension products. The intercalating agent may be any intercalating agent as ethidium bromide, dihydroethidium, ethidium homodimer-1, ethidium homodimer-2, acridine, propidium iodide, YOYO-1 and TOTO-1. When the intercalating agent is ethidium bromide the concn. is about 4 to 7 mg/mL, preferable 5 mg/mL. The invention also provides a reaction extension mixt. reagent, wherein the reagent comprises: a polymg. enzyme, dNTPs, a buffer, an intercalating agent as for example ethidium bromide. The reaction extension mixt. reagent may be a PCR reaction mixt. or anyone medium which an extension reaction of nucleic acid was made. .