Reference

Solubilization of protein-dye complexes on nitrocellulose to quantify proteins spectrophotometrically

Solubilization of protein-dye complexes on nitrocellulose to quantify proteins spectrophotometrically. Goldring, J. P. Dean; Ravaioli, Lillian (Department of Biochemistry, University of the Witwatersrand, Johannesburg, S. Afr.). Analytical Biochemistry, 242(2), 197-201 (English) 1996 Academic. CODEN: ANBCA2. ISSN: 0003-2697. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Proteins absorbed directly onto nitrocellulose membranes were stained with amido black, ponceau S, colloidal silver, or Coomassie blue and solubilized in DMSO and the absorbance was measured spectrophotometrically. The optimal wavelength of each dye/protein/nitrocellulose soln. was found to be at 625, 529, 420, and 600 nm, resp. A linear relation was found between the protein concn. and absorbance at the appropriate wavelength for all the stains with individual purified proteins or protein mixts. Protein (0.2-0.8 mg) can be detd. with the colloidal silver and 2-30 mg with the other stains.There are some commonly used reagents with their cas registry numbers 6226-79-5 and 67-68-5 in this article. Coomassie blue produced variable background staining of the nitrocellulose and is therefore not recommended. Proteins transferred electrophoretically to nitrocellulose from a SDS-polyacrylamide gel were also stained with the above dyes and solubilized in DMSO. Amido black was the most sensitive stain, detecting proteins in the range of 1-10 mg. Components of the gel interfered with silver staining. .

System for staining fungi and protozoa based on presence of chitin in microorganisms

All Rights Reserved. System for staining fungi and protozoa based on presence of chitin in microorganisms. Dao, My Lien (University of South Florida, USA). U.S. Pat. Appl. Publ. US 2007031917 A1 8 Feb 2007, 15pp., Cont.-in-part of U.There are some reagents with their cas registry numbers 6226-79-5 and 64-19-7 are used in this study.S. Ser. No. 249,663. (English). (United States of America). CODEN: USXXCO. INCL: 435034000. APPLICATION: US 2006-537665 2 Oct 2006. PRIORITY: US 2002-319214P 29 Apr 2002; US 2003-249663 29 Apr 2003. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 10 The present invention provides a method for the staining of fungi and microsporidia for observation with a light microscope based upon the presence of chitin in the compn. of these organisms. With the method of the present invention a sample to be analyzed is treated with a soln. of Ponceau S and Stains-all dye. The sample is then selectively decolorized and rinsed. The resulting sample is examd. with a light microscope, or photographed for a permanent record, to identify the presence of a variety of microorganisms, to include fungi and microsporidia. .