Detail of "62683-29-8"

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Effect of 12-O-tetradecanoylphorbol 13-acetate on the production of colony-stimulating factor from murine marrow reticular cell line (H-1)

Effect of 12-O-tetradecanoylphorbol 13-acetate on the production of colony-stimulating factor from murine marrow reticular cell line (H-1). Nakamura, Masato; Harigaya, Kenichi; Watanabe, Yonosuke (Sch. Med., Keio Univ., Tokyo, Japan). Igaku no Ayumi, 127(10), 991-3 (Japanese) 1983. CODEN: IGAYAY. ISSN: 0367-7826. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) The mechanism of the tumor-promoting action of 12-O-tetradecanoylphorbol 13-acetate (I) [16561-29-8] on the proliferation and differentiation of bone marrow cells was studied using the bone marrow reticular cell line (H-1) of mouse. The H-1 cells were incubated with 10-9-10-2M I at 33° for 3 days. The supernatant was dialyzed through a Spectrapor-1 membrane (pore size corresponding to mol. wt. of 6000-8000), filtered through a Gelman membrane (pore size 0.2 m), and assayed for potency of colony-stimulating factor (CSF) [62683-29-8] using mouse(C57BL/6) bone marrow cells. I (10-7-10-5M) significantly enhanced the release of CSF during culture of H-1 cells, but not the proliferation rate of H-1 cells. The addn. of I had no influence on the CSF assay. The tumor-promoting effect of I on mononuclear granulocyte precursors is likely caused by an increased formation of CSF by coexisting bone marrow cells.

Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium

Clonal growth of human acute myeloid leukemia cells (ML-1 and HL-60) in serum-free agar medium. Taketazu, Fumitoshi; Kubota, Kazuo; Kajigaya, Sachiko; Shionoya, Shigeru; Motoyoshi, Kazuo; Saito, Masaki; Takaku, Fumimaro; Miura, Yasusada (Inst. Hematol., Jichi Med. Sch., Tochigi 329-04, Japan). Cancer Res., 44(2), 531-5 (English) 1984. CODEN: CNREA8. ISSN: 0008-5472. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 15 Human acute myeloid leukemia (ML-1 and HL-60) cells grew continuously in the serum-free liq. medium supplemented with human transferrin and bovine insulin. Both ML-1 and HL-60 cells formed clusters and colonies in the serum-free agar medium supplemented with bovine serum albumin, human transferrin, cholesterol, and L-a-phosphatidylcholine. Medium conditioned by phytohemagglutinin-stimulated leukocytes prepd. in the absence of serum had 3 types of colony-stimulating factor [62683-29-8] on normal human bone marrow cells. When fetal calf serum was present, medium conditioned by phytohemagglutinin-stimulated leukocytes stimulated the clonal growth of HL-60 cells at the lower concn. However, it inhibited that of ML-1 cells. In contrast, under serum-free conditions, medium conditioned by phytohemagglutinin-stimulated leukocytes promoted the clonal growth of both ML-1 and HL-60 cells at the lower concns. The study using a Sephadex G-200 column revealed that, in the serum-supplemented cultures, HL-60 cells responded to 1 of 3 colony-stimulating factors and to an activity with mol. wt. ~12,000, while ML-1 cells responded only to an activity with mol. wt. ~12,000. In the serum-free cultures, both ML-1 and HL-60 cells were stimulated by activities with mol. wts. of 62,000 and 54,000, resp. Thus, the detn. of growth factors for cell lines is dependent on culture conditions, particularly on serum components. There is a heterogeneity of ML-1 and HL-60 cells in response to the growth factors. There is potential importance of demonstration of heterogeneity among different cell lines in establishing requirements for different stages of differentiation.