Reference

Metabolism of amino acids in Ascaridia galli: transamination

Metabolism of amino acids in Ascaridia galli: transamination. Singh, Govind; Srivastava, V. M.In this study， 61-90-5 and 63-68-3 are also used. L. (Div. Biochem., Cent. Drug Res. Inst., Lucknow 226001, India). Z. Parasitenkd., 69(6), 783-8 (English) 1983. CODEN: ZEPAA6. ISSN: 0044-3255. DOCUMENT TYPE: Journal CA Section: 12 (Nonmammalian Biochemistry) A. galli, Using 2-oxoglutaric acid as an acceptor, transaminates alanine and aspartic acid at high rates. Among other amino acids valine, phenylalanine, leucine, isoleucine, arginine, tyrosine, and methionine are metabolized at moderate rates, whereas lysine, serine, threonine, cysteine, glycine, histidine, tryptophan, DOPA, and GABA appear to be inert in this respect. Body parts mimic the whole worm in the pattern of transamination of various amino acids with the exception of methionine. Alanine, aspartic acid, and glutamic acid may transfer their amino groups also to pyruvate and oxalacetate. Alanine and aspartate transaminases are located mainly in the cytosol and mitochondrial fractions. .

Tissue metabolism of methionine in sheep

Tissue metabolism of methionine in sheep. Benevenga, N. J.; Radcliffe, B. C.; Egan, A. R. (Waite Agric. Res. Inst., Univ. Adelaide, Glen Osmond 5064, Australia).There are some reagents with their cas registry numbers 63-68-3 and 583-92-6 are used in this study. Aust. J. Biol. Sci., 36(5-6), 475-85 (English) 1983. CODEN: AJBSAM. ISSN: 0004-9417. DOCUMENT TYPE: Journal CA Section: 13 (Mammalian Biochemistry) The rate of oxidn. of the carboxyl and Me groups of [14C]methionine to CO2 by homogenates of liver, kidney cortex, pancreas, muscle, and small intestinal mucosa was studied in 2 breeds of sheep (Merino and Poll Dorset Horn) at 2 wk, 3 mo, and 4 yr of age. Na a-oxo-g-methiolbutyrate (0.4 mM) stimulated prodn. of CO2 from the carboxyl C of methionine, but not from the Me C. Na pyruvate did not affect the recovery of CO2 from either the carboxyl or Me of methionine. HCO2Na (15 mM) suppressed the conversion of the Me C of methioniine to CO2 by liver and kidney homogenates to 4% and 50%, resp., of control values, but did not affect the percentage of carboxyl C of methionine recovered in CO2 with either tissue. With addn. of S-methyl-L-cysteine (40 mM) and 3-methylthiopropionate (10 mM), the percentage of Me and carboxyl C recovered in CO2 was reduced to ~20% of control values in homogenates of both tissues. Activity per g of tissue was higher in liver and kidney cortex than in pancreas, intestinal mucosa, or muscle, with no significant differences due to breed (merino or Poll Dorset Horn) or sex (ewe, ram, or wether) of sheep. Conversion of both the carboxyl and Me C to CO2 by liver was significantly lower in 2-wk-old lambs than in older animals. The activity in other tissues was not markedly affected by age. Results are discussed in relation to evidence of alternative pathways of methionine catabolism, and capacities of the tissues of the sheep to catabolize methionine by alternative pathways. .