Detail of "64183-27-3"

Reference

Use of nucleotide analogs by class I and class II CCA-adding enzymes (tRNA nucleotidyltransferase): Deciphering the basis for nucleotide selection

Use of nucleotide analogs by class I and class II CCA-adding enzymes (tRNA nucleotidyltransferase): Deciphering the basis for nucleotide selection. Cho, Hyundae D.; Oyelere, Adegboyega K.; Strobel, Scott A.Chemicals with cas numbers 64183-27-3 and 56-65-5 also play role.; Weiner, Alan M. (Department of Biochemistry, School of Medicine, University of Washington, Seattle, WA 98195-7350, USA). RNA, 9(8), 970-981 (English) 2003 Cold Spring Harbor Laboratory Press. CODEN: RNARFU. ISSN: 1355-8382. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) We explored the specificity and nature of the nucleotide-binding pocket of the CCA-adding enzyme (tRNA nucleotidyltransferase) by using CTP and ATP analogs as substrates for a panel of class I and class II enzymes. Overall, class I and class II enzymes displayed remarkably similar substrate requirements, implying that the mechanism of CCA addn. is conserved between enzyme classes despite the absence of obvious sequence homol. outside the active site signature sequence. CTP substrates are more tolerant of base modifications than ATP substrates, but sugar modifications prevent incorporation of both CTP and ATP analogs by class I and class II enzymes. Use of CTP analogs (zebularine, pseudoisocytidine, 6-azacytidine, but not 6-azauridine) suggests that base modifications generally do not interfere with recognition or incorporation of CTP analogs by either class I or class II enzymes, and that UTP is excluded because N-3 is a pos. determinant and/or O-4 is an antideterminant. Use of ATP analogs (N6-methyladenosine, diaminopurine, purine, 2-aminopurine, and 7-deaza-adenosine, but not guanosine, deoxyadenosine, 2'-O-methyladenosine, 2'-deoxy-2'-fluoroadenosine, or inosine) suggests that base modifications generally do not interfere with recognition or incorporation of ATP analogs by either class I or class II enzymes, and that GTP is excluded because N-1 is a pos. determinant and/or the 2-amino and 6-keto groups are antideterminants. We also found that the 3'-terminal sequence of the growing tRNA substrate can affect the efficiency or specificity of subsequent nucleotide addn. Our data set should allow rigorous evaluation of structural hypotheses for nucleotide selection based on existing and future crystal structures. .

Structure of 2'-deoxy-2'-fluoroadenosine (dAfl): C10H12N5O3F

Structure of 2'-deoxy-2'-fluoroadenosine (dAfl): C10H12N5O3F. Morishita, Kaoru; Hakoshima, Toshio; Fujiwara, Takaji; Tomita, Kenichi; Kaneyasu, Toshinori; Uesugi, Seiichi; Ikehara, Morio (Fac. Pharm. Sci., Osaka Univ., Suita 565, Japan). Acta Crystallogr., Sect. C: Cryst. Struct. Commun., C40(3), 434-6 (English) 1984. CODEN: ACSCEE. ISSN: 0108-2701. DOCUMENT TYPE: Journal CA Section: 75 (Crystallography and Liquid Crystals) Section cross-reference(s): 33 The title compd. is monoclinic, space group P21, with a 4.791(2), b 10.428(2), c 11.507(2) ?, and b = 102.06(1)°; d.(exptl.) = 1.576(4) and d.(calcd.) = 1.590 for Z = 2. Final R = 0.066 for 1066 reflections.In this experiment, several chemicals are used like 64183-27-3 At. parameters are given. The base orientation around the glycosidic bond is anti, and the conformation around the exocyclic C(4')-C(5') bond is gauche-trans. The sugar ring has C(3')-endo (3E) puckering. .