Detail of "64849-39-4"

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Enzymic transglucosylation of rubusoside and the structure-sweetness relationship of steviol-bisglycosides

Enzymic transglucosylation of rubusoside and the structure-sweetness relationship of steviol-bisglycosides. Darise, Muchsin; Mizutani, Kenji; Kasai, Ryoji; Tanaka, Osamu; Kitahata, Sumio; Okada, Shigetaka; Ogawa, Susumu; Murakami, Fumikazu; Chen, Feng Huai (Sch. Med., Hiroshima Univ., Hiroshima 734, Japan). Agric. Biol. Chem., 48(10), 2483-8 (English) 1984. CODEN: ABCHA6. ISSN: 0002-1369. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) Rubusoside [64849-39-4], the b-D-glucosyl ester of 13-O-b-D-glucosylsteviol which was isolated from leaves of Rubus suavissimus collected in China as the major sweet principle (yield: 5.4%), was subjected to a-1,4 transglucosylation with the cyclodextrin glucosyltransferase [9030-09-5] produced by Bacillus megaterius strain no. 5 using sol. starch as a donor. A significant improvement in the quality of sweetness was obsd. for the crude reaction mixt., which was sepd. into mono-, di-, tri-, tetra-, penta-, and hexa-glucosylated products. All isomers of the mono- and di-glucosylated products were further sepd. Evaluation of the sweetness of these products compared with stevioside and rebaudioside A disclosed that the ratio of the no. of glucose units at the 13-hydroxyl group to that at 19-carboxyl group seems to have a relation with the sweetness as well as the quality of taste for glucosides of this type.

Novel steviol glucosides, their manufacture with b-fructofuranosyltransferase, and their use as sweeteners

Novel steviol glucosides, their manufacture with b-fructofuranosyltransferase, and their use as sweeteners. Ishikawa, Hiroshi; Kitahata, Sumio; Shibata, Tomohiko; Suzuki, Hiroshi; Mikuni, Katsuhiko; Fujita, Takateru; Hara, Kozo (Hokkaido Sugar Co., Ltd.; Osaka City of; Ensuiko Sugar Refining Co., Ltd., Japan). Jpn. Kokai Tokkyo Koho JP 03099092 A2 24 Apr 1991 Heisei, 7 pp. (Japan). CODEN: JKXXAF. CLASS: ICM: C07H015-256. ICS: A23L001-236; C12P019-56.Several reagents with their cas registry numbers 64849-39-4 and 57817-89-7 are used here. ICA: A23L001-30. ICI: C12P019-56, C12R001-06. APPLICATION: JP 89-234675 12 Sep 1989. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Section cross-reference(s): 17, 62, 63 The title compds. I (R = b-D-glucopyranosyl, 2-O-b-D-glucopyranosyl-b-D-glucopyranosyl), useful as sweeteners for foods, dentifrices, mouthwashes, and pharmaceuticals, are manufd. by treatment of aq. solns. or dispersions contg. rubusoside or stevioside and b-fructose-contg. sugars with b-fructofuranosyltransferase of Arthrobacter K-1. Arthrobacter K-1 was shake-cultured in a medium contg. yeast ext., polypeptone, solubilized starch, and salts at 37°, centrifuged, and the supernatant was treated with phosphate buffer contg. stevioside and sucrose at 50° for 16 h to produce I (R = 2-O-b-D-glucopyranosyl-b-D-glucopyranosyl), which showed better sweetness and less bitterness than rebaudioside A. .