Detail of "65455-52-9"

Reference

Studies on the procurement of coagulation factor VIII: selective precipitation of factor VIII with hydrophilic polymers

Studies on the procurement of coagulation factor VIII: selective precipitation of factor VIII with hydrophilic polymers. Farrugia, A.; Griffin, B.; Pepper, D.; Prowse, C. (Edinburgh S-E Scotland Blood Transfus. Serv., R. Infirm., Edinburgh, UK). Thromb. Haemostasis, 51(3), 338-42 (English) 1984. CODEN: THHADQ. ISSN: 0340-6245. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Different concns. and mol. wts. of hydroxyethyl starch [9005-27-0], dextran [9004-54-0], PEG [25322-68-3], PVP [9003-39-8], Ficoll [25702-74-3], Percoll [65455-52-9] and albumin were examd. for their ability to ppt. the blood coagulation factor VIII [9001-27-8] complex from cooled (not frozen) fresh CPD plasma. At optimal concns., near quant. recovery of VIIIR:Ag and VIII:CAg was achieved in 2 h with minimal pptn. of fibrinogen or total protein. The best sepns. were obsd. with hydroxyethyl starch, albumin or Ficoll. PVP and PEG gave inferior purifns. Results for cryoppt. and intermediate-purity factor VIII conc. were inferior to those obtained with plasma. Simple pre-concn. of plasma prior to chilling is an attractive alternative for large scale continuous prodn.

Method for purifying mesenchymal stem cells using gradient centrifugation

Method for purifying mesenchymal stem cells using gradient centrifugation. Lange, Claudia; Zander, Axel Rolf (Universitaetsklinikum Hamburg-Eppendorf, Germany). PCT Int. Appl. WO 2005015151 A2 17 Feb 2005, 29 pp. DESIGNATED STATES: W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW; RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, CY, DE, DK, ES, FI, FR, GA, GB, GR, IE, IT, LU, MC, ML, MR, NE, NL, PT, SE, SN, TD, TG, TR. (German). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: G01N. APPLICATION: WO 2004-EP8865 6 Aug 2004. PRIORITY: DE 2003-10336152 6 Aug 2003. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 15, 63 The invention relates to a method for purifying mesenchymal stem cells, producing cells corresponding to prior art, the cells obtained being characterized by an increased proliferation capacity while maintaining the multipotency and typical antigen characteristics. To this end, mesenchymal stem cells that can be multiplied in a significantly improved manner compared to prior art are first provided and can also be differentiated, after a longer cultivation period, in three mesenchymal directions.Except for chemicals metioned above, 65455-52-9 and 25702-74-3 are also used. .