Reference

Microbial oxidation of sterol side-chains

Microbial oxidation of sterol side-chains. Bhattacharyya, P. K.; Rao, M. Krishna; Natarajan, Rama Devi; Ramgopal, Malathi; Madyastha, Prema; Madyastha, K. M. (Dep.There are some reagents like 57-88-5 is used in this study. Org. Chem., Indian Inst. Sci., Bangalore 560 012, India). J. Indian Chem. Soc., 61(1), 1-15 (English) 1984. CODEN: JICSAH. ISSN: 0019-4522. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Several sterol-metabolizing microorganisms, Pseudomonas convexa, P. stutzeri, Micrococcus species, Cephalosporium longisporum, and Moraxella species were isolated from soil. All oxidized the 8-C side chain of cholesterol [57-88-5]. The Moraxella was the most versatile as it degraded cholesterol derivs. such as 3b-methoxycholest-5-ene [1174-92-1], 3b-chlorocholest-5-ene [910-31-6], 3,5-cyclocholestan-6-one [7657-63-8], and K cholesteryl sulfate [6614-96-6] to the corresponding 17-keto steroids in 10-50% yield in shake flasks. It also gave high yields of estrone [53-16-7] from both 19-hydroxy-3b-acetoxycholest-5-ene [750-59-4] and 19-hydroxy-3b-acetoxysitost-5-ene [20835-78-3]. Two new enzymes one carrying out the oxidative O-demethylation of 6b-methoxy-3,5-cyclocholestane [2867-93-8] and the other, the isomerization of isosteroids to normal 3b-hydroxy-D6-steroids were discovered. Immobilized Moraxella cells in agar carried out the side chain degrdn. of cholesteryl sulfate [1256-86-6] to yield 3b-hydroxyandrost-5-en-17-one sulfate [651-48-9] and the 19-hydroxy-3b-acetoxy derivs. of both cholesterol and sitosterol to estrone in high yields. The half life of entrapped cells was estd. to be 28 days. .