Reference

Removal of heavy-metal toxicity by complexanes

Removal of heavy-metal toxicity by complexanes. I. Effect of complexanes on the accumulation and toxicity in high concentration heavy-metal solutions (cadmium, zinc, copper, and lead) for aquatic animals. Muramoto, Shigeki (Agric. Biol. Lab., Okayama Univ., Kurashiki, Japan). Nogaku Kenkyu, 57(2), 127-42 (Japanese) 1978. CODEN: NOGKAV. ISSN: 0029-0874. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Mortality of carp, Cyprinus carpio, in metal salt solns. with added complexing agents [EDTA [60-00-4], NTA [139-13-9], or DTPA [67-43-6]] was obsd. for 2 days. The decreasing order of toxicity was Cu > Cd > Zn > Pb. Complexing agents lowered the toxicity and accumulation of the metals. Increase of pH by addn. of complexing agents (e.g. pH 9.4 for Zn and EDTA) did not affect mortality.

Antidotal effects of chelating agents against cadmium-induced cytotoxicity tested in vitro

Antidotal effects of chelating agents against cadmium-induced cytotoxicity tested in vitro. Fischer, Anna Barbara; Seibold, Georg (Hyg.-Inst., Justus-Liebig-Univ., Giessen, Fed. Rep. Ger.). Heavy Met. Environ., Int. Conf., 5th, Volume 2, 110-12. Edited by: Lekkas, Themistokles D. CEP Consult.: Edinburgh, UK. (English) 1985. CODEN: 55JBAC. DOCUMENT TYPE: Conference CA Section: 4 (Toxicology) The cytotoxicity of 10 chelating agents and their efficacy against Cd was studied in cultures of HeLa cells and Chinese hamster cells B14F28. At up to 10-4M the following agents had no cytotoxic effects: penicillamine (PA) [52-67-5], N-acetylpenicillamine (NAPA) [15537-71-0], mercaptosuccinate (MSA) [70-49-5], meso-2,3-dimercaptosuccinate (DMSA) [304-55-2], DMPS [4076-02-2], nitrilotriacetic acid (NTA) [139-13-9], EDTA [60-00-4], and EGTA [67-42-5]. Diethyldithiocarbamate (DDTC) [147-84-2] and DTPA [67-43-6] inhibited cell proliferation in 2 days by 50% at 10-20 mM, while the median lethal concn. was 30-100 mM for DDTC and 20-40 mM for DTPA. Cellular uptake of Cd was unaffected by PA, NAPA, MSA, DMPS, and NTA. EDTA, EGTA, and DTPA inhibited and DMSA and DTTC stimulated uptake significantly. When cells were concomitantly exposed to 1 mM CdCl2 and 10 mm of the chelators, only EDTa, EGTA, and DTPA were effective against Cd cytotoxicity. Only DDTC caused the efflux of Cd from Cd-treated cultures.