Detail of "674289-55-5"

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Blebbistatin, a Myosin II Inhibitor, Is Photoinactivated by Blue Light

Blebbistatin, a Myosin II Inhibitor, Is Photoinactivated by Blue Light. Sakamoto, Takeshi; Limouze, John; Combs, Christian A.; Straight, Aaron F.; Sellers, James R. (Laboratory of Molecular Cardiology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA). Biochemistry, 44(2), 584-588 (English) 2005 American Chemical Society. CODEN: BICHAW. ISSN: 0006-2960. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) Section cross-reference(s): 13 Blebbistatin is a small mol. inhibitor discovered in a screen for inhibitors of nonmuscle myosin IIA. Blebbistatin inhibits the actin-activated MgATPase activity and in vitro motility of class II myosins. In cells, it has been shown to inhibit contraction of the cytokinetic ring. Blebbistatin has some photochem. properties that may affect its behavior in cells. 674289-55-5 which is the cas registry number of some chemical is mentioned. In particular, we have found that exposure to light at wavelengths below 488 nm rapidly inactivates the inhibitory action of blebbistatin using the in vitro motility of myosin as an assay. In addn., the inhibition of cytokinetic ring contraction can be reversed by exposure of the cells to blue light. This property may be useful in locally reversing the action of blebbistatin treatment in a cell. However, caution should be exercised as free radicals may be produced upon irradn. of blebbistatin that could result in cell damage. .