Detail of "6779-87-9"

Reference

Biopterin production by enzymic reduction

Biopterin production by enzymic reduction. (Suntory, Ltd., Japan). Jpn. Kokai Tokkyo Koho JP 59082091 A2 11 May 1984 Showa, 5 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C12P017-18. APPLICATION: JP 82-190160 29 Oct 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) L-7,8-Dihydrobiopterin (I) [6779-87-9] is reduced by dihydrofolic acid reductase [9002-03-3] and mixed with L-ascorbic acid and L-cysteine.HCl to yield 6-(R)-L-erythro-5,6,7,8-tetrahydrobiopterin (II) [17528-72-2]. Thus, 15 g biopterin [22150-76-1] dissolved in 255 mL 2N NaOH was mixed with 255 mL H2O and 16.35 g Zn powder. The mixt. was stirred under N2 for 2 h, passed through Sephadex G-25, and the column washed with 150 mL water. The effluent and washing were combined and adjusted to pH 5.8 with 78 mL 50% AcOH to yield I. To the soln. were added 57 g glucose 6-phosphate in 300 mL water, 1 g NADPH in 100 mL 5% NaOAc, 10,000 units glucose 6-phosphate reductase in 30 mL water, and water to a total vol. of 1.5 L. Under Ar atm., 2000 units of dihydrofolic acid reductase in 20 mL 10 mM phosphate buffer (pH 5.6) were added. The mixt. was stirred under Ar atm. at 37° for 14 h and mixed with L-ascorbic acid 15 and L-cysteine.HCl 7.5 g. The mixt. was passed through an activated C column. The column was washed with 15 L soln. contg. L-ascorbic acid 0.1 and L-cysteine HCl 0.05% and eluted with 20 L Me2CO-vitamin C mixt. (4:1). The eluant was concd. to 1 L and filtered. The filtrate was worked up by column chromatog. on CM-Sephadex C-25, with 0.25% vitamin C as eluant, to yield II (150 mg).

Participation of pterins in the control of lymphocyte stimulation and lymphoblast proliferation

Participation of pterins in the control of lymphocyte stimulation and lymphoblast proliferation. Ziegler, Irmgard; Hamm, Ulrike; Berndt, J. (Inst. Toxikol. Biochem., Ges. Strahlen- Umweltforsch., Munich D-8000/2, Fed. Rep. 6779-87-9 and 487-21-8 are cas registry numbers. These chemicals are also mentioned in this article. Ger.). Cancer Res., 43(11), 5356-9 (English) 1983. CODEN: CNREA8. ISSN: 0008-5472. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) Among 13 major intermediates of protein metab. and 2 lumazines, xanthopterin (but not dihydroxanthopterin) inhibited cell proliferation (half-max. inhibition at 1.8 ′ 10-5 M) during Con A-induced lymphocyte activation in prestimulated lymphocytes and in a lymphoid cell line grown in continuous culture (LS-2). LS-2 cells exposed to max. inhibitor concns. largely maintained the initial thymidine incorporation rate for about 40 h but failed to enter logarithmic growth. Isoxanthopterin inhibition was found only in serum-free medium, since it is trapped by the a-acid glycoprotein present in the serum. The reduced biopterin derivs., sepiapterin, dihydrobiopterin, and tetrahydrobiopterin, were costimulators during Con A-induced lymphocyte activation. Their costimulatory effect followed an optimum curve and peaked at 1.5 ′ 10-5-3 ′ 10-5 M. It was highest at the suboptimal and supraoptimal Con A concn. The D-erythro isomer dihydroneopterin was inactive. Thus, the anabolic-reduced biopterin derivs. are not simply lymphocytic products, but in combination with the catabolites xanthopterin and isoxanthopterin, also participate in the regulation of lymphocyte activation. Hence, they fulfill the criteria for lymphokines. .