Reference

Chlorpromazine and dithioerythritol protection against acute ethanol toxicity

Chlorpromazine and dithioerythritol protection against acute ethanol toxicity. Gailis, L.; Tourigny, A. (Inst. Cardiol. Quebec, Laval Univ., Quebec, PQ, Can.). Alcohol.: Clin. Exp. Res., 8(3), 308-13 (English) 1984. CODEN: ACRSDM. ISSN: 0145-6008. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) The effect of 2 substances that decrease body temp. [chlorpromazine (CPZ) [50-53-3] and dithioerythritol (DTE) [6892-68-8]] on EtOH [64-17-5] toxicity was studied. Matched groups of 10 mice were injected s.c. with CPZ (5 mg/kg), DTE (80 mg/kg), or saline (controls). CPZ and DTE significantly depressed the rectal temp. to 32.8 and 34.5°, resp. One hour later, all 3 groups received a specified dose of EtOH (6.5-10.7 g/kg, 24% wt./vol., i.p.). The expt. was repeated 17 times at different EtOH doses. The pretreatments increased the 1 h LD50 from 7.8 g/kg for the controls to 8.6 g/kg (DTE) and 10.0 g/kg (CPZ). The protective effect of CPZ was maximal at ~5 mg/kg, and less at both lower and higher CPZ doses. When the temp. drop was prevented by directly heating the mice, the protective effect of DTE could be eliminated, but the effect of CPZ was only partially prevented. Placing the CPZ-treated mice in a warmer environment only produced a major reversal of CPZ protection when rectal temp. reached 38°. Thus, CPZ and DTE decrease body temp. and protect against acute EtOH toxicity. The protection seems to be due at least partially to the decrease in body temp. prior to EtOH injection.

Evidence for the involvement of essential sulfhydryl group in rat hepatic lactogenic receptor but not in somatogenic receptor

Evidence for the involvement of essential sulfhydryl group in rat hepatic lactogenic receptor but not in somatogenic receptor. Tsim, Karl W. K.; Cheng, Christopher H. K. (Dep. Biochem., Chin. Univ. Hong Kong, Shatin, Hong Kong). Mol. Cell. Endocrinol., 38(1), 61-6 (English) 1984. CODEN: MCEND6. ISSN: 0303-7207. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Incorporation of p-chloromercuribenzene sulfonate (PCMBS) (1mM) in the assay medium for rat hepatic lactogenic receptor produced complete inhibition of binding of 125I-labeled ovine prolactin (oPRL) [9002-62-4] to the membrane. However, the presence of the thiol-reactive agent produced no effect on the binding of 125I-labeled bovine growth hormone (bGH) [9002-72-6] to rat hepatic somatogenic receptor. Pretreatment of rat hepatic membranes with PCMBS inhibited the binding of [125I]oPRL but not that of [125I]bGH. The lactogenic receptor binding inhibition by PCMBS pretreatment was both concn.- and time-dependent, with complete inhibition at 0.5 mM for 60 min at 0°. Scatchard anal. of [125I]oPRL binding to membranes at 50% inhibition by PCMBS (0.11 mM) revealed that the binding capacity was decreased rather than the binding affinity. Furthermore, the inhibition of lactogenic receptor binding by PCMBS was reversed by dithioerythritol (DTE) [6892-68-8] treatment. Following 80% inhibition by 0.2 mM PCMBS, full recovery of receptor binding was achieved at 6 mM DTE for 60 min at 0°. The recovered membrane showed no difference from the control membrane in terms of binding capacity and affinity.