Detail of "69168-45-2"

Reference

Photosensitization to ultraviolet irradiation and selective killing of cells following uptake of pyrene-containing fatty acid

Photosensitization to ultraviolet irradiation and selective killing of cells following uptake of pyrene-containing fatty acid. Fibach, Eitan; Morand, Olivier; Gatt, Shimon (Hadassah Univ. Hosp., Hebrew Univ., Jerusalem, Israel). J. Cell Sci., 85, 149-59 (English) 1986. CODEN: JNCSAI. ISSN: 0021-9533. DOCUMENT TYPE: Journal CA Section: 8 (Radiation Biochemistry) Section cross-reference(s): 14 Human promyelocytic leukemia and murine erythroleukemia cells were incubated with 12-(1-pyrene)dodecanoic acid (P12), a long-chain fatty acid to which a pyrene ring has been attached covalently. This acid was transported across the plasma membranes of cells and subsequently incorporated into their neutral lipids and phospholipids. Irradn. of these pyrene-contg. cells for short periods (0.5-4 min) with UV light at 366 nm resulted in eventual cell death. Similar irradn.Several substances with their cas registry numbers 3073-59-4 and 69168-45-2 may be metioned in this study. had no effect on cells that had not been exposed to P12. The time of the period of irradn. necessary for inducing the toxic process was related to the quantity of P12 incorporated, the latter being a function of the resp. metabolic activity of the individual cell type. The degree of incorporation of P12 into a cell, and consequently its acquired sensitivity to killing by UV irradn. at 366 nm, was affected by the incubation temp. and addn. of nonfluorescent fatty acid, albumin, or other serum proteins. Different degrees of incorporation of P12 into various cell types were used for selective killing and elimination of cell populations by irradn. at 366 nm. The combined procedure of preincubation with P12 followed by UV irradn. thus permitted selection of cell types with a greater resistance to this procedure. .

Induction of lipid storage in cultured leukemic myeloid cells by pyrene-dodecanoic acid

Induction of lipid storage in cultured leukemic myeloid cells by pyrene-dodecanoic acid. Morand, Olivier; Fibach, Eitan; Livni, Nelly; Gatt, Shimon (Lab. Neurochim., Hop. Salpetriere, Paris 75651/13, Fr.). Biochim. Biophys. Acta, 793(1), 95-104 (English) 1984. CODEN: BBACAQ. ISSN: 0006-3002. DOCUMENT TYPE: Journal CA Section: 14 (Mammalian Pathological Biochemistry) When incubated for 1-3 days in the presence of the fatty acid analog 12-(1-pyrene)dodecanoic acid, the neutral lipid content of cultured human leukemic myeloid cells increased considerably, while that of the phospholipids increased to a much lesser extent. Among the neutral lipids, di- and monoacylglycerols predominated and a considerable portion of the fatty acyl residues of these newly synthesized neutral lipids consisted of pyrene-dodecanoic acid. Light microscopy showed evidence for the presence of highly fluorescent lipid droplets within the cells. Electron microscopy showed lipid globules, mostly devoid of a unit membrane, multivesicular inclusion bodies, and some multilamellar membranous structures. In comparison, cells incubated with palmitic acid show neither these cellular structures, nor the increase of the neutral lipid content. The lipid storage, induced by pyrene-dodecanoic acid, is probably related to ineffective degrdn.In this experiment, several chemicals are used like 69168-45-2 of this fatty acid analog and might serve as an exptl. model of cellular lipidosis. .