Reference

Reversed-phase high-performance liquid chromatographic determination of ginsenosides of Panax quinquefolium

Reversed-phase high-performance liquid chromatographic determination of ginsenosides of Panax quinquefolium. Court, William A.; Hendel, John G.; Elmi, Jama (Chemistry Laboratory, Agriculture and Agri-Food Canada, Pest Management Research Centre, P.O. Box 186, Delhi, ON N4B 2W9, Can.). Journal of Chromatography, A, 755(1), 11-17 (English) 1996 Elsevier. CODEN: JCRAEY. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) A reversed-phase HPLC detn. of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1, Ro, gypenoside XVII, pseudoginsenoside-F11, and an indirect detn. of the malonyl-ginsenosides Rb1, Rb2, Rc and Rd in Panax quinquefolium was developed. Ground ginseng samples were extd. with aq. methanol in an ultrasonic bath. The neutral triterpene saponins were detd. directly by liq. chromatog. on the ext. The acidic ginsenosides were detd. indirectly following the hydrolysis with aq. potassium hydroxide.In this experiment, several chemicals are used like 69884-00-0 and 80321-69-3 Sepns. were achieved with a phosphate buffer-acetonitrile gradient system using a C18 reversed-phase column. .

Differentiation of ginsenoside Rf and pseudoginsenoside F11 by in-source collision-induced dissociation high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry

All Rights Reserved. Differentiation of ginsenoside Rf and pseudoginsenoside F11 by in-source collision-induced dissociation high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry. Ma, Xiaoqiong; Xiao, Hongbin; Liang, Xinmiao (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Peop. Rep. China). Fenxi Huaxue, 34(9), 1273-1277 (Chinese) 2006 Kexue Chubanshe. 52286-58-5 and 69884-00-0 are cas registry numbers. These chemicals are also mentioned in this article. CODEN: FHHHDT. ISSN: 0253-3820. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Ginsenoside Rf and pseudoginsenoside F11, two chem. markers of Panax ginseng and Panax quinquefolium, were analyzed using in-source collision-induced dissocn. high performance liq. chromatog.-atm. pressure chem. ionization-mass spectrometry (HPLC-APCI/MS) method. Under the gradient elution reversed-phase liq. chromatog. and in-source collision-induced dissocn. conditions, aglycon and deglycoslyated ions of ginsenoside Rf and pseudoginsenoside F11 were obviously obsd. From the differences of these ions, the two isomer compds. were identified. The detection limit of ginsenoside Rf and pseudoginsenoside F11 is about 10-7 g on column. Panax ginseng and Panax quinquefolium can be distinguished in a single HPLC- APCI/MS expt. rapidly by this way. .