Reference

2,4-Diaminophenol ether couplers for oxidation hair dye

2,4-Diaminophenol ether couplers for oxidation hair dye. Rose, David; Lieske, Edgar (Henkel K.Several substances are used for example 96-41-3 which is its cas registry number.-G.a.A., Fed. Rep. Ger.). Ger. Offen. DE 3313905 A1 25 Oct 1984, 19 pp. (German). (Germany). CODEN: GWXXBX. CLASS: IC: C07C093-14; A61K007-13. APPLICATION: DE 83-3313905 16 Apr 1983. DOCUMENT TYPE: Patent CA Section: 41 (Dyes, Organic Pigments, Fluorescent Brighteners, and Photographic Sensitizers) Section cross-reference(s): 62 The title compds. (I; R = H, C1-4 alkyl; n = 4-11; ￡3 R groups = alkyl) are prepd. and used as couplers of low toxicity for producing (with known developers) deep blue to red-brown shades of high fastness on human hair. Thus, reaction of cyclopentanol [96-41-3] with 2,4-(O2N)2C6H3F [70-34-8] in Et3N-DMF at 20° for 5 days gave 1-(cyclopentyloxy)-2,4-dinitrobenzene [94650-03-0] which was hydrogenated over Pd to give I (R = H, n = 4) (II) [94650-08-5]. Other I were prepd. by the same method. Formulations contg. II and p-tolylenediamine, when oxidized with H2O2 produced bluish black shades on untreated gray hair. .

Derivatization of primary amines by 2-naphthalenesulfonyl chloride for high-performance liquid chromatographic assay of neomycin sulfate

Derivatization of primary amines by 2-naphthalenesulfonyl chloride for high-performance liquid chromatographic assay of neomycin sulfate. Tsuji, Kiyoshi; Jenkins, Kevin M. (Control Res. Dev., Upjohn Co., Kalamazoo, MI 49001, USA). J. Chromatogr., 369(1), 105-15 (English) 1986. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) A normal phase HPLC method was developed for the assay of neomycin [1404-04-2]. The method involves precolumn derivatization with 2-naphthalenesulfonyl chloride (I) [93-11-8] followed by extn. in CHCl3 and chromatog. using a normal phase silica column with detection at 254 nm. The std. curve for the HPLC assay of neomycin is linear with a correlation coeff. of 0.9996 over the range of 0.02 to 0.4 mg/mL. Neomycin B [119-04-0], and C [66-86-4], and neamine [3947-65-7] can be sepd. and quantified isocratically with relative std. deviations of 0.92% and 1.4% for neomycin (B + 1/2C) and neamine, resp. Prednisolone is used as an internal std. to aid in quantification. Mass spectrometric data confirms that neomycin is derivatized at all the six primary amines on the neomycin mol. Eight lots of neomycin were used to compare the HPLC [I and 1-fluoro-2,4-dinitrobenzene (DNFB) [70-34-8]], gas-liq. chromatog. and microbiol. assay methods. The av. results of the I-HPLC method fell between those of the microbiol. and DNFB-HPLC methods. Also, good correlation of the neomycin C contents in neomycin were obtained between the I-HPLC and DNFB-HPLC methods.