Reference

4-(Aminomethyl)cyclohexane carboxylic acid

4-(Aminomethyl)cyclohexane carboxylic acid. 92279-48-6 is also in the experiment. (Showa Denko K. K., Japan). Jpn. Kokai Tokkyo Koho JP 59080784 A2 10 May 1984 Showa, 4 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C25B003-00. APPLICATION: JP 82-188244 28 Oct 1982. DOCUMENT TYPE: Patent CA Section: 72 (Electrochemistry) Section cross-reference(s): 24 In an electrolytic cell having a cation-exchanging membrane, an aq. soln. contg. alkali metal 4-(aminomethyl)cyclohexanecarboxylate and an aq. caustic alkali soln. are supplied to the anode chamber and the cathode chamber, resp., and electrolyzed. The above method manufs. at a low cost and by simple operations 4-(aminomethyl)cyclohexanecarboxylic acid (I) [701-54-2], which is useful as raw material for medicine and polymers. Thus, an aq. soln. of Na 4-(aminomethyl)cyclohexanecarboxylate and an aq. NaOH soln. were supplied to an anode chamber and a cathode chamber, resp., in an electrolytic vessel sepd. by Nafion 390 (a cation-exchange membrane), and electrolyzed to form I (yield 75.8%). .

Genetic engineering of Streptomyces hygroscopicus and other microbes for production of polyketides and other natural products

Genetic engineering of Streptomyces hygroscopicus and other microbes for production of polyketides and other natural products. Gregory, Matthew Alan; Gaisser, Sabine; Petkovic, Hrvoje; Moss, Steven (Biotica Technology Limited, UK). PCT Int. Appl. WO 2004007709 A2 22 Jan 2004, 216 pp. DESIGNATED STATES: W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW, AM, AZ, BY, KG, KZ, MD, RU; RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, CY, DE, DK, ES, FI, FR, GA, GB, GR, IE, IT, LU, MC, ML, MR, NE, NL, PT, SE, SN, TD, TG, TR. (English). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: C12N015-00. APPLICATION: WO 2003-GB3230 16 Jul 2003. PRIORITY: GB 2002-16509 16 Jul 2002; GB 2002-24922 25 Oct 2002. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Section cross-reference(s): 3, 63 The present invention relates to prodn. of polyketides and other natural products and to libraries of compds. and individual novel compds. One important area is the isolation and potential use of novel FKBP-ligand analogs and host cells that produce these compds. The invention is particularly concerned with methods for the efficient transformation of microbial strains that produce FKBP analogs and recombinant cells in which cloned genes or gene cassettes are expressed to generate novel compds. such as polyketide (esp. rapamycin) FK520- and FK506-binding protein-ligand analogs, and to processes for their prepn., and to means employed therein (e.g. nucleic acids, vectors, gene cassettes and genetically modified strains). 95233-12-8 and 701-54-2 which are cas registry numbers of substances are two of reagents here. Thus, a conjugative deletion plasmid is electroporated in dam-dcm- ET12567 Escherichia coli strain, which is then conjugated with spores from Streptomyces hygroscopicus to result in deletions of the rapamycin modifying genes rapQ, rapO/N, rapM, rapL, rapK, rapJ, and rapI. Genes may be restated by complementation. The S. hygroscopicus mutants are incubated with natural or non-natural starter units to produce a variety of rapamycin and FKBP-ligand analogs. .