Reference

Magnetic resonance of a monoclonal anti-spin-label antibody

Magnetic resonance of a monoclonal anti-spin-label antibody. Anglister, Jacob; Frey, Tom; McConnell, Harden M. (Stauffer Lab. Phys. Chem., Stanford Univ., Stanford, CA 94305, USA). Biochemistry, 23(6), 1138-42 (English) 1984. CODEN: BICHAW. ISSN: 0006-2960. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) The NMR spectra of monoclonal Fab antibody fragments have been recorded in the absence and presence of a specific spin-labeled hapten [dinitrophenyl (DNP)]. The difference spectra reveal the presence of 11 arom. amino acids in the region of the combining site. By selective deuteration of this hybridoma antibody, these amino acids have been identified as 3 tryptophans, 6 tyrosines, 1 phenylalanine, and 1 histidine. Difference spectra have also been recorded that depend on ring-current chem. shifts. The latter difference spectra show that among these 11 amino acids there are 2 tyrosines and 1 tryptophan in close proximity to the DNP ring. Based on UV absorption measurements, it is concluded that this tryptophan is stacked against the DNP ring. 63-91-2 and 73-22-3 are just another two chemicals used in this study. Selective deuteration of hybridoma antibodies directed against a paramagnetic hapten provides a powerful new approach for the study of the structural basis of antibody diversity and specificity. .

Carrier for the fixation of microorganisms

Carrier for the fixation of microorganisms. Geirnaert, Gilles (Argiles et Mineraux A.G.S. S. A., Fr.). Eur. Pat. Appl. EP 93027 A1 2 Nov 1983, 14 pp. DESIGNATED STATES: R: CH, DE, GB, LI, NL, SE. (French). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: C04B035-18; C12N011-14; C12N005-00. APPLICATION: EP 83-400596 23 Mar 1983. PRIORITY: FR 82-7205 27 Apr 1982; FR 82-15845 21 Sep 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) A ceramic support for living cells contg. ~10% Mg is made from kaolinitic clay and talc [14807-96-6]. Thus, 70 part clay and 30 parts talc were heated at 1350°, then ground and sifted to retain particles of 100-200 mm. The particles were suspended with Escherichia coli at pH 9, then placed in a column, and a soln.In this experiment, several chemicals are used like 73-22-3 and 7439-95-4 contg. indole was passed through the column at an undisclosed rate. The conversion of indole to tryptophan [73-22-3] was ~60% for nearly 1 mo. .