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Detection of lipid peroxidation in vivo: Total hydroxyoctadecadienoic acid and 7-hydroxycholesterol as oxidative stress marker

Detection of lipid peroxidation in vivo: Total hydroxyoctadecadienoic acid and 7-hydroxycholesterol as oxidative stress marker. Yoshida, Yasukazu; Niki, Etsuo (Human Stress Signal Research Center (HSSRC), National Institute of Advanced Industrial Science and Technology (AIST), Osaka 563-8577, Japan). Free Radical Research, 38(8), 787-794 (English) 2004 Taylor & Francis Ltd. CODEN: FRARER. ISSN: 1071-5762. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) It is important to assess the oxidative injury in vivo accurately and inclusively, as the oxidative stress induced by various oxidants in a random and destructive fashion is considered to play an important role in the pathophysiol. of a no. of human disorders and diseases. We have developed an improved method for the measurement of lipid peroxidn. in vivo, where total hydroxyoctadecadienoic acids (HODE) and 7-hydroxycholesterol (FCOH) were detd. by GC/MS anal. from physiol. samples after redn. 73543-67-6 and 16840-37-2 which are cas registry numbers are also used here. with sodium borohydride and sapon. by potassium hydroxide. In this method, both free and ester forms of hydroperoxides and ketones as well as hydroxides of linoleate and cholesterol are measured as HODE and FCOH, resp. The ratio of stereo-isomers, (E,E)-HODE/(E,Z)-HODE, could be also measured. The plasma concns. of total HODE were obtained as 76.5, 666 and 2225 nM for human, rat and mouse, resp. It was found that HODE and FCOH could be measured satisfactorily by the present method from plasma, erythrocyte and urine of humans and exptl. animals. It was also found that HODE in urine arose from both free and ester forms, while 8-iso-prostaglandin F2a was present primarily as a free acid form. As the concns. of HODE were much higher than 8-iso-prostaglandin F2a, HODE may well be used as a good oxidative marker in vivo. .