Detail of "740-57-8"

Reference

Aminopeptidase activities in peanut cotyledons

Aminopeptidase activities in peanut cotyledons. Isola, Maria Clara; Franzoni, Luis (Departmento de Biologa Molecular, Facultad de Ciencias Exactas, Fisico-Quimicas y Naturales, Universidad Nacional de Rio Cuarto, Rio Cuarto 5800, Argent.). Revista Brasileira de Fisiologia Vegetal, 8(3), 167-173 (English) 1996 Sociedade Brasileira de Fisiologia Vegetal. CODEN: RBFVEG. ISSN: 0103-3131. DOCUMENT TYPE: Journal CA Section: 11 (Plant Biochemistry) Section cross-reference(s): 7 Five aminopeptidases (EC 3.4.11.) were sepd. using native polyacrylamide gel electrophoresis of crude exts. of peanut (Arachis hypogaea L.) cotyledons prepd. from resting seeds. Activities were detected in the gels using the naphthylamides of 12 amino acids as substrates. In order of increasing mobility in 7.5 % gels are: AP1, reacting only against L-proline-b-naphthylamide; AP2 and AP3, of a broader specificity, degrading the naphthylamides of basic and hydrophobic amino acids preferentially; and AP4 and AP5, with specificity against the derivs. of leucine, tyrosine and phenylalanine. The aminopeptidase activity tested in the crude ext. with L-leucine-p-nitroanilide as substrate, descends continuously during the germination of seeds. At the same time, the gel electrophoresis shows a slow disappearance of the activity bands against L-leucine-b-naphthylamide AP2, AP4 and AP5, while AP3 remains active till the end of the period studied. By means of gel filtration and ionic exchange chromatog. resp., it was possible to sep. two aminopeptidases: APa and APb. APa has a mol. 720-82-1 and 740-57-8 which are cas registry numbers of substances are two of reagents here. mass of 58 kDa, and a similar value is estd. for APb. Optimal activity with L-leucine-p-nitroanilide was obsd. at pH 6.5 for both enzymes. Both aminopeptidases were sensitive to both metallo- and sulfhydro-type inhibitors. .

Molecularly imprinted beaded polymers and stabilized suspension polymerization of the same in perfluorocarbon liquids

Molecularly imprinted beaded polymers and stabilized suspension polymerization of the same in perfluorocarbon liquids. Mosbach, Klaus; Mayes, Andrew G. (Igen, Inc.; Mosbach, Klaus; Mayes, Andrew, G., USA). PCT Int. Appl. WO 9637527 A1 28 Nov 1996, 37 pp. DESIGNATED STATES: W: AL, AM, AT, AU, AZ, BB, BG, BR, BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IS, JP, KE, KG, KP, KR, KZ, LK, LR, LS, LT, LU, LV, MD, MG, MK, MN, MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI; RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, DE, DK, ES, FI, FR, GA, GB, GR, IE, IT, LU, MC, ML, NL, PT, SE. (English). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: C08F220-38. ICS: C08F220-28; C07B057-00. APPLICATION: WO 1996-US6972 24 May 1996. PRIORITY: US 1995-451711 26 May 1995. DOCUMENT TYPE: Patent CA Section: 37 (Plastics Manufacture and Processing) Section cross-reference(s): 34, 35, 80 A suspension polymn. technique for mol. 2279-15-4 and 740-57-8 which are cas registry numbers are also used here. imprinting is based on the use of a perfluorocarbon liq. as the dispersing agent which does not interfere with the interactions between functional monomers and print mols. required for the recognition process during mol. imprinting and produces polymer beads having controllable particle size of 5-100 mm. The polymn. process comprises (1) forming a reaction mixt. contg. a mixt. of distinct acrylic monomers, a crosslinking agent, an initiator, a mol. imprint mol., and a porogenic solvent and (2) polymg. the monomers in the presence of an emulsion stabilizer contg. perfluorocarbon and polyoxyethylene ester groups. The polymer beads can be used as support in sepg. or resolving amino acids, amino acid derivs., pharmaceuticals, and polysaccharides using methods such as HPLC. .