Detail of "7411-49-6"

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Contrast enhancement of the brownish horseradish peroxidase-activated 3,3'-diaminobenzidine tetrahydrochloride reaction product in black and white photomicrography by the use of interference filters

Contrast enhancement of the brownish horseradish peroxidase-activated 3,3'-diaminobenzidine tetrahydrochloride reaction product in black and white photomicrography by the use of interference filters. Moeller, M.; Glistrup, O. V.; Olsen, W. (Inst. Med. Anat., Department B, Univ. Copenhagen, Copenhagen, Den.). J.Several reagents with their cas registry numbers 7411-49-6 and 9003-99-0 are used here. Histochem. Cytochem., 32(1), 37-42 (English) 1984. CODEN: JHCYAS. ISSN: 0022-1554. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) An interference filter combination for contrast enhancement of the brownish, peroxidase-activated, 3,3'-diaminobenzidine tetrahydrochloride reaction product was developed. The filter combination allows transmission of light in 2 narrow bands, representing those of the primary colors green (480-500 nm) and red (575-585 nm) as well as a broader band between 385-430 nm. The visual contrast enhancement of horseradish peroxidase-contg. neurons caused by the interference filters was excellent. In particular, intracellular details were prominent in the presence of the interference filters compared with different blue absorption filters. The contrast enhancement was evaluated by microdensitometry of an Agfapan 25 emulsion onto which peroxidase-contg. neurons in noncounterstained sections were exposed. The microdensitometry showed a neuron to background contrast enhancement of 30% compared to neutral gray filters and of 11% compared to a Kodak Wratten 80A blue absorption filter. The interference filter principle can be used for contrast enhancement of other compd. colors. .

Signal pathway profiling of prostate cancer using reverse phase protein arrays

Signal pathway profiling of prostate cancer using reverse phase protein arrays. Grubb, Robert L.; Calvert, Valerie S.; Wulkuhle, Julia D.; Paweletz, Cloud P.; Linehan, W. Marston; Phillips, John L.; Chuaqui, Rodrigo; Valasco, Alfredo; Gillespie, John; Emmert-buck, Michael; Liotta, Lance A.; Petricoin, Emanuel F. (Urologic Oncology Branch, National Cancer Institute, Bethesda, MD, USA). Proteomics, 3(11), 2142-2146 (English) 2003 Wiley-VCH Verlag GmbH & Co.There are some reagents with their cas registry numbers 7411-49-6 and 9004-70-0 are used in this study. KGaA. CODEN: PROTC7. ISSN: 1615-9853. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 14 Reverse phase protein arrays represent a new proteomics microarray technol. with which to study the fluctuating state of the proteome in minute quantities of cells. The activation status of cell signaling pathways controls cellular fate and deregulation of these pathways underpins carcinogenesis. Changes in pathway activation that occur between early stage prostatic epithelial lesions, prostatic stroma and the extracellular matrix can be analyzed by obtaining pure populations of cell types by laser capture microdissection (LCM) and analyzing the relative states of several key phosphorylation points within the cellular circuitry. We have applied reverse phase protein array technol. to analyze the status of key points in cell signaling involved in pro-survival, mitogenic, apoptotic and growth regulation pathways in the progression from normal prostate epithelium to invasive prostate cancer. Using multiplexed reverse phase protein arrays coupled with LCM, the states of signaling changes during disease progression from prostate cancer study sets were analyzed. Focused anal. of phospho-specific endpoints revealed changes in cellular signaling events through disease progression and between patients. We have used a new protein array technol. to study specific mol. pathways believed to be important in cell survival and progression from normal epithelium to invasive carcinoma directly from human tissue specimens. With the advent of mol. targeted therapeutics, the identification, characterization and monitoring of the signaling events within actual human biopsies will be crit. for patient-tailored therapy. .