Reference

A simple procedure for enzyme labeling of progesterone derivatives: application to active esters formed using N,N'-disuccinimidyl carbonate

A simple procedure for enzyme labeling of progesterone derivatives: application to active esters formed using N,N'-disuccinimidyl carbonate. Sauer, M.Several substances are used for example 57-83-0 and 74124-79-1 which are their cas registry numbers. J.; Morris, B. A. (Cattle Breed. Cent., Minist. Agric., Fish. Food, Shinfield/Reading RG2 9BZ, UK). J. Steroid Biochem., 26(1), 165-7 (English) 1987. CODEN: JSTBBK. ISSN: 0022-4731. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 32 N,N'-Disuccinimidyl carbonate [74124-79-1] was used to synthesize N-hydroxysuccinimide esters of 11a-hydroxyprogesterone 11-[1,4-14C]hemisuccinate (P11-HS) and 11a-hydroxprogesterone 11-glucuronide (P11-Glu) in a one-step procedure at room temp. Enzyme-labeled progesterone was subsequently formed by reaction of the ester, without purifn., with alk. phosphatase. Labels produced by this simple procedure compared favorably with those formed using an established method of ester synthesis when assessed in enzyme immunoassay (EIA). .

Synthesis, anti-HIV and anti-proliferative activity of new urea and nitrosourea derivatives of amino acids

Synthesis, anti-HIV and anti-proliferative activity of new urea and nitrosourea derivatives of amino acids. Gallant, G.; Salvador, R.; Dulude, H. (Fac. Pharm., Univ. Montreal, Montreal, PQ H3C 3J7, Can.). Antiviral Chem., 2(5), 313-19 (English) 1991. 2450-71-7 and 74124-79-1 which are cas registry numbers are also used here. CODEN: ACCHEH. ISSN: 0956-3202. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Section cross-reference(s): 27 A series of N-Me, N-(2-chloroethyl) and N-propargyl urea and nitrosourea derivs. of amino acids were synthesized and tested for anti-HIV and anti-proliferative activity. All the agents tested showed only a weak ability to counteract the cytopathic effects of the HIV-1 virus on a T4 lymphocyte cell line (CEM-IW). At high concn., the N-Me and N-propargyl ureas were cytotoxic. The nitrosoureas failed to suppress cell proliferation of uninfected CEM-IW cells. The lack of activity and cytotoxicity of the nitrosoureas in this model could be explained by their short chem. half-life. .