Detail of "74424-56-9"

CAS Number:
74424-56-9
Name:
L-Asparagine,N-[O-2-(acetylamino)-2-deoxy-b-D-glucopyranosyl-(1®4)-O-[O-2-(acetylamino)-2-deoxy-b-D-glucopyranosyl-(1®2)-a-D-mannopyranosyl-(1®3)]-O-[O-a-D-mannopyranosyl-(1®3)-O-[a-D-mannopyranosyl-(1®6)]-a-D-mannopyranosyl-(1®6)]-O-b-D-mannopyranosyl-(1®4)-O-2-(acetylamino)-2-deoxy-b-D-glucopyranosyl-(1®4)-2-(acetylamino)-2-deoxy-b-D-glucopyranosyl]- (9CI)
Molecular Structure:
Formula:
C66H110 N6 O48
Molecular Weight:
0
Synonyms:
Man(5)GlcNAc(4)Asn

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Resolution of some glycopeptides of hen ovalbumin by reverse-phase high-pressure liquid chromatography: Resolution of some glycopeptides of hen ovalbumin by reverse-phase high-pressure liquid chromatography. Dua, Virendra K.; Bush, C. Allen (Dep. Chem., Illinois Inst.Chemicals with cas numbers 38784-68-8 and 74424-56-9 also play role. Technol., Chicago, IL 60616, USA). Anal. Biochem., 137(1), 33-40 (English) 1984. CODEN: ANBCA2. ISSN: 0003-2697. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) The carbohydrate heterogeneity of hen ovalbumin high-mannose glycopeptides was resolved by reversed-phase high-performance liq. chromatog. with UV detection, following pronase digestion of ovalbumin and sepn. of glycopeptides by chromatog. on Dowex AG-50W-X2, and the purity and structure of the glycopeptides was assessed by 1H-NMR. HPLC was carried out on a RP reversed-phase column equipped with a silica gel precolumn and elution was with phosphate buffer (1 mM, pH 6.0). Two of the compds. were identified as the mannose-5 and -6 glycopeptides (E3 and D3) and the third as the mannose-6 glycopeptide substituted by two N-acetyl glucosamine (GlcNAc) residues (C3B). The NMR spectra and anal. chromatograms of the glycopeptides show high purity and freedom from contaminants previously identified in ovalbumin glycopeptides. .