Detail of "7493-95-0"

Reference

Purification and some properties of a-galactosidase from Pseudomonas fluorescens H-601

Purification and some properties of a-galactosidase from Pseudomonas fluorescens H-601. Hashimoto, Hiroyuki; Goto, Masaru; Katayama, Chie; Kitahata, Sumio (Osaka Munic. Tech. Res. Inst., Osaka 536, Japan). Agric. Biol. Chem., 55(11), 2831-8 (English) 1991. CODEN: ABCHA6. ISSN: 0002-1369. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) P. fluorescens H-601, isolated from soil, produces a-galactosidase. The enzyme was purified to homogeneity by disc electrophoresis after column chromatogs. on Butyl-Toyopearl 650M, DEAE-Toyopearl 650M, and Toyopearl HW-55F. The enzyme had a mol. wt. of 390,000 by gel filtration with Toyopearl HW-55F and 86,000 by SDS-PAGE and an isoelec. point of 6.3. The enzyme was most active at pH 6.There are some commonly used reagents with their cas registry numbers 19887-85-5 and 7493-95-0 in this article.0-7.0 and at 45° and stable up to 40° at pH 6.5 for 15 min of incubation. The enzyme hydrolyzed p-nitrophenyl a-galactoside, o-nitrophenyl a-galactoside, melibiose, raffinose, and stachyose at the relative velocities of 100, 59, 28, 13, and 12. The enzyme had strong transfer activity and wide acceptor specificity. .

Glycosidase-catalyzed synthesis of di- and trisaccharide derivatives related to antigens involved in the hyperacute rejection of xenotransplants

Glycosidase-catalyzed synthesis of di- and trisaccharide derivatives related to antigens involved in the hyperacute rejection of xenotransplants. Nilsson, Kurt G. I. (Glycorex AB, Lund S-223 70, Swed.). Tetrahedron Letters, 38(1), 133-136 (English) 1997 Elsevier. CODEN: TELEAY. ISSN: 0040-4039. DOCUMENT TYPE: Journal CA Section: 33 (Carbohydrates) Section cross-reference(s): 9, 15 A convenient enzymic procedure, suitable for large scale prepn. of the b-thioethyl 2-N-Teoc-deriv. of Gala1-3Galb1-4GlcNAc, is described (Teoc = 2,2,2-trichloroethoxycarbonyl). b-D-Galactosidase from Bullera singularis was used for the specific synthesis of Galb1-4GlcNTeocb-SEt from lactose and D-GlcNTeocb-SEt. a-D-Galactosidase from coffee beans was used for the stereospecific and highly regioselective prepn.In this experiment, several chemicals are used like 7493-95-0 and 186665-14-5 of Gala1-3Galb1-4GlcNTeocb-SEt, employing D-Gala-OPhNO2-p as glycosyl donor and Galb1-4GlcNTeocb-SEt as acceptor. .