Detail of "75168-11-5"

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Capillary electrophoresis of cardiolipin with on-line dye interaction and spectrophotometric detection

Capillary electrophoresis of cardiolipin with on-line dye interaction and spectrophotometric detection. Qi, Lining; Danielson, Neil D.; Dai, Qiang; Lee, Ray M. (Chemistry and Biochemistry Department, Miami University, Oxford, OH 45056, USA). Electrophoresis, 24(10), 1680-1686 (English) 2003 Wiley-VCH Verlag GmbH & Co. KGaA. CODEN: ELCTDN. ISSN: 0173-0835. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Cardiolipin is an important phospholipid present in the mitochondrial inner membrane. It plays a key function in mitochondrial respiration by interacting with many enzymes or cofactors related to oxidative phosphorylation complexes. We have detd. the concn. of cardiolipin using online 10-N-nonyl acridine orange (NAO) dye interaction capillary electrophoresis (CE) and spectrophotometric detection with a sample throughput of 3 min. In addn. to the presence of 0.1 mM NAO, the background electrolyte (BGE) compn. has been set at 80% methanol-10% acetonitrile-10% H2O (all vol./vol.) to provide both good soly. and the max. absorbance enhancement at 497 nm for the NAO-cardiolipin complex as compared to NAO alone. Sample consumption for each injection is about 57 nL. A calibration curve is established from 0.5 mM to 0.1 mM with R2 = 0.9912 with a detection limit of 0.05 mM for cardiolipin. In a blind study, actual mitochondrial cell membrane samples in the mL range before or after UV light exposure were analyzed using the CE method. Cardiolipin concn. decreased in the different parts of the membrane sample upon UV photolysis of the cells. Support for the theory that UV light can induce cardiolipin translocation from the inner membrane (IM) to the outer membrane (OM) was indicated by a significant percentage increase of cardiolipin (as measured by the cardiolipin in the OM as compared to the sum total in the OM and IM) from 30.7±2.4% before UV light photolysis to 38. 75168-11-5 is just another one chemical used in this study.3±2.2% after UV irradn. .

Direct Sampling from Muscle Cross Sections for Electrophoretic Analysis of Individual Mitochondria

Direct Sampling from Muscle Cross Sections for Electrophoretic Analysis of Individual Mitochondria.Some chemicals with cas registry numbers like 9002-07-7 and 75168-11-5 are also used. Ahmadzadeh, Hossein; Johnson, Ryan D.; Thompson, LaDora; Arriaga, Edgar A. (Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA). Analytical Chemistry, 76(2), 315-321 (English) 2004 American Chemical Society. CODEN: ANCHAM. ISSN: 0003-2700. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 6, 13 Muscle is a highly heterogeneous tissue. Practical approaches to sample selectively small regions of muscle cross sections would help to effectively utilize anal. techniques on muscle studies while taking into account tissue heterogeneity. In this report, semimembranosus muscle tissue cross sections were directly sampled and analyzed by capillary electrophoresis (CE) with laser-induced fluorescence detection (LIF). Prior to CE-LIF anal., a small region in the muscle cross section was stained with 10-nonyl acridine orange (NAO) which is a mitochondrion-selective fluorescent probe known to form a stable complex with cardiolipin, a phospholipid found only in mitochondria. By micromanipulation, the injection end of the capillary was brought into contact with the tissue exhibiting fluorescently labeled mitochondria. Sampling from a region similar in size to the cross section of a single fiber was carried out by applying 11 kPa of neg. pressure for 3 s. When an elec. field of -200V/cm was applied, fluorescently labeled mitochondria electromigrated and were individually detected by postcolumn LIF detection. For each sample, the electropherogram displays a migration time window with a collection of narrow peaks. The collection of individual peak measurements is represented as a distribution of individual intensities related to cardiolipin content of mitochondria and a distribution of individual electrophoretic mobilities. Positioning the capillary injection end was sufficiently spatially accurate to deplete mitochondria in the sampled region upon repetitive injections. Treatment of a muscle cross section with a protease (trypsin) prior to mitochondria sampling resulted in a higher no. of detected mitochondria, suggesting that one of the effects of this enzyme is a partial digestion of the muscles myofibrils, which eases the release of interfibrillar mitochondria entangled within these fibers. The protease treatment also resulted in changes to the electrophoretic mobility distribution of individual mitochondria, which may imply that partial digestion of proteins bound to the mitochondria contributes to the alteration in the electrophoretic mobility of mitochondria. The ability to sample a region as small as a single muscle fiber cross section and its direct CE-LIF anal. opens exciting possibilities for the direct anal. of muscle biopsies and mapping the mitochondrial electrophoretic properties in highly heterogeneous tissues. .