Detail of "75976-10-2"

Reference

Pancreatic polypeptide response to exercise

Pancreatic polypeptide response to exercise. Effect of alanine and glucose ingestion in carbohydrate-starved athletes. Koeslag, J. H.; Levinrad, L. I.; Klaff, L. J. (Metrop. Sports Sci. Cent., Univ. Cape Town, Rondebosch, S. Afr.). SAMJ, 67(22), 884-7 (English) 1985. CODEN: SAMJEJ. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 13 Human pancreatic polypeptide (hPP) [75976-10-2] secretion in response to 90 min of exercise before and after the ingestion of 50 g glucose [50-99-7] or L-alanine [56-41-7] were examd. in carbohydrate-starved athletes. There was a marked increase in plasma hPP concn. with exercise under thes circumstances, despite previous reports that the hPP response to exercise is eliminated by training. Glucose and alanine ingestion had no effect on hPP either before or after exercise. This differentiates hPP from other hormones in which secretion is increased by exercise in carbohydrate-depleted subjects.

Molecular characterization of a second mouse pancreatic polypeptide receptor and its inactivated human homolog

Molecular characterization of a second mouse pancreatic polypeptide receptor and its inactivated human homolog. Gregor, Paul; Feng, Yun; DeCarr, Lynn B.; Cornfield, Linda J.; McCaleb, Michael L. (Metabolic Disorders Res., Bayer Corp., West Haven, CT 06516, USA). Journal of Biological Chemistry, 271(44), 27776-27781 (English) 1996 American Society for Biochemistry and Molecular Biology. CODEN: JBCHA3. ISSN: 0021-9258. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 3 The family of mammalian neuropeptide Y (NPY)/peptide YY (PYY)/pancreatic polypeptide (PP) receptors comprises several G protein-coupled receptors, i.e Y1, Y2, and Y4/PP1. The authors now report cloning of a novel member of this family named PP2. The coding region of the mouse PP2 gene reveals no introns and predicts a seven transmembrane domain (TM) receptor of 371 amino acids. Percent identities of the mouse PP2 to mouse Y1, mouse Y4/PP1 and human Y2 receptors are 53, 42, and 31, resp. The mouse PP2 receptor expressed in COS cells binds rat 125I-PP with high affinity, i.e. IC50 = 65 pM. Pharmacol.Several substances with their cas registry numbers 75976-10-2 and 183469-70-7 may be metioned in this study. characterization of 125I-PP binding shows a rank order of potency of PP ? PYY 3 NPY, which is similar to that of the mouse Y4/PP1 receptor. Mouse PP2 transcripts were not detectable by Northern anal. in adult tissues and in 11-, 15-, and 17-day-old embryos. However, a 9.8-kb PP2 transcript was detectable in 7-day-old mouse embryo, i.e. prior to the organogenesis of pancreas and the onset of PP prodn. The authors have also cloned the human homolog of PP2, which is a single copy gene and maps to human chromosome 5q31. Surprisingly, the human PP2 cDNAs and gene sequences display a single base deletion in the coding region. This frameshift mutation predicts a truncated receptor of 290 amino acids without TM7. Transfection of COS-7 cells with several different human PP2 expression constructs failed to confirm any specific binding of 125I-PP, 125I-PYY, or 125I-NPY to cell membranes. These data suggest that in mouse there are at least two PP receptors, Y4/PP1 and PP2, whereas in humans, PP2 is either functionally inactive or it has acquired a PP-independent function. .