Reference

Measurement of low angiotensin concentrations after ethanol and Dowex extraction procedures

Measurement of low angiotensin concentrations after ethanol and Dowex extraction procedures. Nussberger, Juerg; Brunner, Dorette B.; Waeber, Bernard; Brunner, Hans R. (Div. Nephrol. Hypertens., Cent. Hosp. 82924-03-6 and 76095-16-4 are also occured in this study. Univ., Lausanne 1011, Switz.). J. Lab. Clin. Med., 103(2), 304-12 (English) 1984. CODEN: JLCMAK. ISSN: 0022-2143. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 1 Current radioimmunoassays do not demonstrate total absence of angiotensin II [11128-99-7] during angiotensin I-converting enzyme [9015-82-1] inhibition. To assess the meaning of plasma angiotensin II detns. during converting enzyme inhibition, plasma angiotensin I [9041-90-1] and II levels of normotensive humans during maximal converting enzyme inhibition by single oral doses of CGS 13945 [82924-03-6], MK 421 [76095-16-4], or MK 521 [76547-98-3] were compared with those of anephric rats (18 h after nephrectomy) after i.v. administration of MK 422 [76420-72-9] (1 mg/kg). Prior to radioimmunoassay, plasma was extd. with Dowex for angiotensin II and blood extd. with EtOH for angiotensin I. During converting enzyme inhibition, in the 20 normotensive subjects plasma angiotensin II was 6.3 pg/mL and blood angiotensin I was 65 pg/mL. In the nephrectomized rats, plasma angiotensin II was 8.9 pg/mL without converting enzyme inhibitor and 7.6 pg/mL with MK 422, and blood angiotensin I was 9.8 pg/mL and 8.2 pg/mL, resp. Dowex extn. of Tris buffer contg. no angiotensin II provided blank values of 5.0-7.8 pg/mL. Thus, plasma angiotensin II of normotensive humans treated with converting enzyme inhibitors fell to blank levels even in the presence of markedly elevated plasma angiotensin I. Angiotensin II concns. in anephric rats with or without converting enzyme inhibition were the same. Plasma levels of angiotensin II <8 pg/mL measured after Dowex extn. probably reflect complete converting enzyme inhibition and virtual absence of angiotensin II generation. .

Enalapril (MK421) and its lysine analog (MK521): a comparison of acute and chronic effects on blood pressure, renin-angiotensin system and sodium excretion in normal man

Enalapril (MK421) and its lysine analog (MK521): a comparison of acute and chronic effects on blood pressure, renin-angiotensin system and sodium excretion in normal man. Hodsman, G. P.; Zabludowski, J. R.; Zoccali, C.; Fraser, R.; Morton, J.Chemicals with cas numbers 7440-23-5 and 75847-73-3 also play role. J.; Murray, G. D.; Robertson, J. I. S. (Western Infirm., MRC, Glasgow G11 6NT, UK). Br. J. Clin. Pharmacol., 17(3), 233-41 (English) 1984. CODEN: BCPHBM. ISSN: 0306-5251. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The immediate and long-term effects of enalapril (MK421)(I) [75847-73-3] and its lysine analog MK521 (II) [76547-98-3] in once-daily dosage, were compared in a study of normal subjects. Both compds. lowered blood pressure equally throughout 24 h without causing tachycardia. The biochem. changes with MK521 were more sustained than with MK421, but this did not affect the magnitude of blood-pressure redn. Twenty-four h after the previous dose, with both active drugs, plasma renin [9015-94-5] concn. was significantly higher on day 8 than on day 1, though angiotensin I did not increase in proportion; this probably reflects a fall in renin-substrate with prolonged converting-enzyme inhibition. There was an early natriuresis with each compd., but this effect was no longer apparent after 8 days of continuous therapy. Both MK421 and MK521 were well tolerated, with no serious side effects. .