Detail of "76877-33-3"

Reference

Differential behavior of the two free sulfhydryl groups of human plasma fibronectin: effects of environmental factors

Differential behavior of the two free sulfhydryl groups of human plasma fibronectin: effects of environmental factors. Narasimhan, C.; Lai, Ching San (Dep. Radiol., Med. Coll. Wisconsin, Milwaukee, WI 53226, USA). Biopolymers, 31(10), 1159-70 (English) 1991. CODEN: BIPMAA. ISSN: 0006-3525. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) A novel approach to label specifically one of the two cryptic, free sulfhydryl groups per subunit of human plasma fibronectin with either an 15N,2H-maleimide spin label or a coumarinylphenyl maleimide fluorescent label is reported. This permits the use of ESR or fluorescence techniques to study mol. dynamics of fibronectin with the label attached to a single site per chain on the protein mol. The method is based on the observation that upon adsorption of fibronectin to a gelatin-coated surface, the SH1 site, located between the DNA-binding and the cell-binding domains, is partially exposed, while the SH2 site, located within the carboxyl-terminal fibrin-binding domain, remains buried and unreactive. The procedures for the prepn. of the selectively labeled fibronectins are described in detail. The physicochem. properties of these single-site labeled fibronectins, particularly as affected by high salt, heparin, surface binding, and temp., were characterized by ESR spin-label and steady-state fluorescence techniques. The steady-state fluorescence measurement indicates that both local environments of SH1 and SH2 sites are relatively hydrophobic, and that the SH2 site is more hydrophobic than the SH1 site. The ESR results show that heparin or high salt induces an increase in the domain flexibility in both SH1 and SH2 regions, perhaps through the disruption of domain-domain interactions in the fibronectin mol., and that the former is more effective than the latter in producing such an effect. The obsd. heparin effect is reversible by addn.There are some commonly used reagents with their cas registry numbers 9005-49-6 and 76877-33-3 in this article. of calcium ions in the SH2 regions but not in the SH1 regions. In addn., at temps. above 44°, both type III homologous regions contg. the free sulfhydryl groups are shown to undergo denaturation and aggregation processes. These data suggest that the newly developed method for differential labeling of the free sulfhydryl groups in fibronectin should be useful for mapping the spatial arrangement of structural domains in the protein mol. using spin-label-spin-probe and fluorescence energy transfer techniques. .

Preparation of a fluorochrome for thiols: N-4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide (CPM)

Preparation of a fluorochrome for thiols: N-4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide (CPM). Stjernloef, P. (Foersvarets Forskningsanst., Umea, Swed.). Report, FAO-C-40210-C3; Order No. PB85-213056, 27 pp. Avail. NTIS From: Gov. Rep. Announce.In this article, certain chemicals are used. One of their cas registry numbers is 76877-33-3 Index (U. S.) 1985, 85(20), Abstr. No. 544,940 (Swedish) 1985. DOCUMENT TYPE: Report CA Section: 27 (Heterocyclic Compounds (One Hetero Atom)) Section cross-reference(s): 7 CPM (I) was prepd. by a 4-step synthesis. The total yield was ~10%. I is for example used in microfluorometric detns. of acetylcholinesterase. .