Detail of "7776-48-9"

Reference

Isomerizing L-glucose to L-fructose

Isomerizing L-glucose to L-fructose. Horwath, Robert O.; Colonna, William J. (Nabisco Brands, Inc., USA). U.S. US 4463093 A 31 Jul 1984, 4 pp. (English). (United States of America). CODEN: USXXAM. CLASS: IC: C12P019-24; C12N009-92; C12R001-72. NCL: 435094000. APPLICATION: US 82-393846 30 Jun 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) L-Fructose [7776-48-9] is produced from L-glucose [921-60-8] by xylose isomerase [9023-82-9] from Candida. A mixt. of 50 mmol L-glucose, 5 mM MnCl2, and 50 mg enzyme is incubated at 37° for 12-18 h, until equil. is reached. The mixt. is then heated and centrifuged to remove denatured protein.

Oxidizing L-sorbitol to L-fructose

Oxidizing L-sorbitol to L-fructose. Horwath, Robert O.; Colonna, William J. (Nabisco Brands, Inc., USA). U.S. US 4467033 A 21 Aug 1984, 6 pp. (English). (United States of America). CODEN: USXXAM. CLASS: IC: C12P019-02; C12N015-00; C12N009-04. NCL: 435105000. APPLICATION: US 82-393847 30 Jun 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) L-Fructose [7776-48-9] is enzymically produced from L-sorbitol [6706-59-8] using D-iditol dehydrogenase (IDH) [9028-22-2] from Pseudomonas. P. fluorescens Is mutagenized0with UV light and grown in a medium contg. salts and 0.5% glucose (no inducer is added). The cells are harvested by centrifugation, disrupted in 0.02M phosphate buffer (pH 7.4), and centrifuged. The supernatant is the cell-free ext. contg. IDH. L-Sorbitol is oxidized in a reaction mixt. contg. 1 mmol L-sorbitol, 50 mL of 25 mM phosphate buffer (pH 8), 3 mmol NAD, methylene blue and cell-free ext. (20-50 mg protein). The mixt. is incubated at 30° for 2-3 h or until equil. is established, heated in a boiling water bath, and the denatured protein removed by centrifugation.