Reference

Effect of vitamin A deficiency on the testis-specific histone TH2B of the rat

Effect of vitamin A deficiency on the testis-specific histone TH2B of the rat. Wattanaseree, Jittaporn; Svasti, Jisnuson; Bubpaniroj, Pim; Mitranond, Viroj (Fac. Sci., Mahidol Univ., Bangkok 10400, Thailand). J. Biochem. (Tokyo), 95(1), 179-86 (English) 1984. CODEN: JOBIAO.Several reagents with their cas registry numbers 68-26-8 and 11103-57-4 are used here. ISSN: 0021-924X. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) The effect of vitamin A [68-26-8] deficiency on rat testis was studied biochem. and histol. The earliest change in nuclear basic proteins was a decrease and disappearance of the testis-specific protein (TP); the disappearance approx. coincided with cessation of growth. At later stages of deficiency, the testis-specific histone TH2B was markedly reduced and in some cases disappeared, as did the TH1 histone band. Histol. studies indicated that the loss of TH2B and TH1 requires a substantial degree of testicular degeneration, because they could still be detected to some extent in testes contg. mainly Sertoli cells, reduced nos. of spermatogonia, and very few spermatocytes. The effect of retinol [68-26-8] in maintaining the germ cells and the testis-specific basic proteins TP and TH2B was very specific in that it could not be replaced by retinoic acid [302-79-4], which can maintain normal body growth. Prolonged supplementation of vitamin A-deficient animals with retinyl palmitate [79-81-2] partially restored the levels of TP, TH2B, and TH1. .

Effect of dietary coconut oil and casein and megadoses of vitamin A or C on tissue lipid peroxidation and hemolysis in vitamin E deficiency

Effect of dietary coconut oil and casein and megadoses of vitamin A or C on tissue lipid peroxidation and hemolysis in vitamin E deficiency. Krishnamurthy, S.; George, T.; Bai, N. Jayanthi (Dep. Biochem., Med. Coll., Trivandrum, India). Acta Vitaminol. Enzymol., 5(3), 165-70 (English) 1983. CODEN: AVEZA6. ISSN: 0300-8924. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) Male rats fed on pellet diet to an av. wt. of 105 g were placed on a vitamin E [1406-18-4] deficient diet contg. 20% coconut oil for 12 wk at 2 dietary protein levels, 20% and 10% casein. Rats on 20% casein diet showed wt. loss, but not so at the 10% casein level. A marked increase in the liver in vitro lipid peroxidn. was obsd. at both protein levels.Several substances with their cas registry numbers 9000-81-1 and 50-81-7 may be metioned in this study. Feeding of retinyl palmitate [79-81-2] at 100,000 IU/100 g body wt. for 4 consecutive days inhibited the liver, brain, and kidney in vitro peroxidn.; megadoses of ascorbic acid [50-81-7] produced less inhibition of the liver peroxidn., but the same degree of inhibition for brain and kidney peroxidn. as in vitamin A-loaded rats. Both dietary protein levels significantly increased H2O2 lysis and this was further enhanced by the large doses of retinyl palmitate or ascorbic acid. Acetylcholine esterase [9000-81-1] and ATPase [9000-83-3], membrane enzymes of erythrocytes, were depressed in all the groups. The glutathione [70-18-8] content of erythrocytes was increased in rats given ascorbic acid. In all the groups, the higher dietary protein levels produced greater loss of body and tissue wts. Thus, a vitamin E-deficient diet supplemented with dietary coconut oil (satd. fat) induces increased in vitro lipid peroxidn. and oxidative lysis of erythrocytes. Megadoses of vitamin A or C suppress the in vitro lipid peroxidn. but enhance the lysis. .